Biology (MindTap Course List)
11th Edition
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Chapter13: Gene Expression
Section13.6: Variations In Gene Expression
Problem 15LO
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Explain the principles of next-generation sequencing and metagenomics
Expert Solution
Principle of next-generation sequencing
Next-generation sequencing
The principle of Next Generation Sequencing is same to Sanger sequencing, which very much depends on capillary electrophoresis. The genomic strand is first fragmented, and then bases in each fragment are identified by emitted signals when these fragments are ligated against a template strand. The Next generation sequencing method use array-based sequencing. Array based sequencing combines the techniques which were developed in Sanger sequencing to process millions of reactions in parallel, resulting in very high speed and throughput at a reduced cost
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