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- Alcohol is considered to be a significant reagent for the isolation of nucleicacids (RNA and DNA). Why is this so?You have purified a virus that infects turnip leaves. Treatment of a sample with phenol removes viral proteins. Application of the residual material to scraped leaves results in the formation of progeny virus particles. You infer that the infectious substance is a nucleic acid. Propose a simple and highly sensitive means of determining whether the infectious nucleic acid is DNA or RNAWith the aid of a diagram, explain the transformation process.
- Explain how you would use the blue-white colony screening technique to identify bacteria containing the recombinant plasmidChoose the combination of answers that most accurately completes the statement.For which of the following would a nucleic acid probe not be used? a. locating a gene on a chromosome b. developing a Southern blot c. identifying a microorganism d. constructing a recombinant plasmidWhat is the function of each reagent used in the extraction of DNA from banana? •Saltwater •Liquid dishwashing soap •Cold isopropyl alcohol
- Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. IF the EcoRI/BamHI double digest produces 3 fragments with only two sizes, what are their sizes? 2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb 500 b and 500 b 3.0 kb 1.0kb and 1.0 kbGive typing answer with explanation and conclusion The use of spin columns with silica for plasmid purification comprises an example of the following purification technique: a. adsorption to a matrix b. non-organic extraction c. organic extraction d. alkaline extraction e. none of the aboveSequence of which of the following cannot be determined using the Maxam Gilbert method?a) Bacteriab) Plantsc) Bacteriophage T7d) Plasmid