Which of the following techniques takes advantage of NET CHARGE to purify/characterize proteins? O Hydrophobic chromatography O Isoelectric focusing O Affinity chromatógraphy O Dialysis
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- Which of the following techniques takes advantage of NET CHARGE to purify/characterize proteins? a. Isoelectric focusing b. Hydrophobic chromatography c. Affinity chromatography d. DialysisExplain.What physical method is most frequently use to determine three dimensional structures of protein? Fluorescence spectroscopy Electron microscopy X ray crystallography Nuclear magnetic resonance spectroscopy Mass spectrometryThe following are denaturing agents for proteins, except:* Please choose one correct answer only. A. Change in pH B. Heat C. Ultraviolet light D. Organic solvents E. None of the given options
- What is the basis for the separation of proteins by the following techniques? (a) gel-filtration chromatography(b) affinity chromatography(c) ion-exchange chromatographyWhich classes of biomolecules would be best separated using normal phase chromatography? Why? steroids mono- and disaccharides flavonoids5.Which method for determining protein structure involves cleaving proteins into smaller polypeptides using enzymes and using performic acid and dithiothreitol (DTT) to remove disulphide bridges? a)Matrix-assisted laser desorption/ionization mass spectrometry b)Electrospray ionization mass spectrometry c)Edman's degradation d)Tandem mass spectrometry e)Enzyme protease fragmentation.
- What is the basis for the separation of proteins by the following techniques? (a) gel-filtration chromatography (b) affinity chromatography (c) ion-exchange chromatography (d) reverse phase HPLCIsoelectric focusing is used to separate …………….. based on their ................ A. salts, solubility B. nucleic acid, size C. proteins, charge D. proteins, size (molecular mass) E. molecules, densityThere can be more than one correct answer Which of the following methods can seperate proteins based on their mass? a.) centrifugation b.) gel filtration chromatography c.) SDS polyacrylamide gel electrophoresis d.) ion exhange chromatography
- Provide a brief definition and a description of the significance of the following terms in NMR spectroscopy (7) intermediate exchange(8) SAR by NMR(9) the Larmour frequencyWhat technique can be used to separate amino acids on the basis of their pI values? (a) Thin-layer chromatography(b) Size-exclusion chromatography(c) Electrophoresis(d) Liquid chromatography(e) Gas chromatographyFour known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?