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- What is the meaning of the word sterile? What would happen if culture mediafreshly prepared were not sterilized? Why should you always waitthe autoclave to drain all the steam (pressure gauge zero position) beforeto open the cover?A cell is 25 μm wide when viewed at 1,000× magnification. Thismeasurement can also be written properly asa. 25 mm. c. 0.025 mm.b. 25,000 mm. d. 2.5 mm.Filtration does/is NOT...?a) Sterilize heat sensitive liquidsb) Kill microbesc) Sterilize providing pores are exceedingly smalld) Used in preparation of vaccines, vitamins, and drugse) Used to capture microbes in ventilation systems via HEPA filters Which of the following is false?a) Pasteurization does not sterilizeb) Ionization radiation is useful for sterilizing food and pharmaceuticals c) Phenolics can be used as antisepticsd) Isopropyl alcohol can sterilize the skine) Steam autoclaving is an effective means of sterilization Which of the following is not a factor that determines the effectiveness of an antimicrobial agent? a) Time of exposure b) Temperature c) pH d) Concentration of agent e) All of the above are factors that determines the effectiveness of an antimicrobial agent 4. Which of the following is not a specific cell target for antimicrobial agents? a) Capsule b) Cell wallc) Cell membraned) DNA / RNA synthesis e) Protein function 5. Which of the following…
- Which specific example of a functional medium would you use to grow microbesfrom the following samples? Explain why it would be appropriate based on itsa. Air sample for bacteria:b. Sample from behind the ear for microbes:c. Sample from table top for fungi:This is a Microbiology question about blood culture analyzers such as the BactecWhat is the process a technician takes if the analyzer alarms for a positive bottle?What action(s) are taken if there are no organisms seen in the Gram Stain made from that bottle?Why are the Methyl Red/Voges-Proskauer tests done after 48 hours of incubation?a) Readings taken at earlier time points may be different from readings taken at 48 hours b) Bacteria need 48 hours to process all the oxygen in the media c) Bacteria grow slowly in the MR/VP media d) K2HPO4 needs 48 hours to react with the acids produced by fermenting bacteria
- Which of the following is used in the sterilization of the medium?a) benmargeb) swabc) autoclaved) centrifugee) coolingCan you give me an example of a specific medium in culture media and fill the information below. General Information Specific a. Name of medium: b. Physical form: c. Category based on formulation: d. Purpose/uses/function: e. List of Ingredients and concentration (per 1000 ml) f. How to prepare?A bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3 and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?
- If a component is heat sensitive, how might you conveniently achieve sterility of that medium? a. filtration b. batch pasteurization c. autoclave and add the isolated component back afterAndrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar. a. What is the total volume of the medium needed? b. What is the amount of each component needed, given the following media composition per liter of distilled water:The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization?3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distille
