Fluorescence measurements of concentration are inherently more sensitive than spectrophotometic (e.g. UV-vis) measurements because:

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter17: Applications Of Infrared Spectrometry
Section: Chapter Questions
Problem 17.13QAP
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Fluorescence measurements of concentration are inherently more sensitive than spectrophotometic (e.g. UV-vis)
measurements because:
Select one:
a. Shorter pathlength cells are used in spectrophotometry
O b. The fluorescence intensity can be better measured because there is no light source
O c. The emergent light is measured at right angles in a fluorimeter, but in a spectrophotometer the emergent beam is
measured in the same direction as the excitation beam.
O d. fluorescent emission depends on the wavelength of light used for excitation whereas for spectrophotometry only the
wavelength of absorption is critical.
O e. Better quality monochromators are used in spectrofluorimeters than in spectrophotometers
O f. The fluorescence intensity can be increased by increasing the incident light intensity (lo), but this has no effect on
absorbance in spectrophotometry.
Transcribed Image Text:Fluorescence measurements of concentration are inherently more sensitive than spectrophotometic (e.g. UV-vis) measurements because: Select one: a. Shorter pathlength cells are used in spectrophotometry O b. The fluorescence intensity can be better measured because there is no light source O c. The emergent light is measured at right angles in a fluorimeter, but in a spectrophotometer the emergent beam is measured in the same direction as the excitation beam. O d. fluorescent emission depends on the wavelength of light used for excitation whereas for spectrophotometry only the wavelength of absorption is critical. O e. Better quality monochromators are used in spectrofluorimeters than in spectrophotometers O f. The fluorescence intensity can be increased by increasing the incident light intensity (lo), but this has no effect on absorbance in spectrophotometry.
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