For Figure 15-7, draw out a series of steps that could explain the origin of this large plasmid containing many transposable elements.

Human Heredity: Principles and Issues (MindTap Course List)
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Chapter13: An Introduction To Genetic Technology
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Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...
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. For Figure 15-7, draw out a series of steps that could explain the origin of this large plasmid containing many
transposable elements.

HIS1 cmR IS50 kan IS50; sm su amp hgIS1
An R plasmid may contain several
transposons carrying resistance genes
-Tn5
-Tn3-
- Tn4-
Resistance-determinant segment
FIGURE 15-7 A schematic map of a plasmid with several insertions of simple and composite
transposons carrying resistance genes. Plasmid sequences are in blue. Genes encoding
resistance to the antibiotics tetracycline (tet"), kanamycin (kan®), streptomycin (sm®),
sulfonamide (suf), and ampicillin (ampR) and to mercury (hg®) are shown. The resistant-
determinant segment can move as a cluster of resistance genes. Tn3 is within Tn4. Each
transposon can be transferred independently. [Data from S. N. Cohen and J. A. Shapiro,
"Transposable Genetic Elements." Copyright 1980 by Scientific American, Inc. All rights reserved.]
Tn10
IS10
Transcribed Image Text:HIS1 cmR IS50 kan IS50; sm su amp hgIS1 An R plasmid may contain several transposons carrying resistance genes -Tn5 -Tn3- - Tn4- Resistance-determinant segment FIGURE 15-7 A schematic map of a plasmid with several insertions of simple and composite transposons carrying resistance genes. Plasmid sequences are in blue. Genes encoding resistance to the antibiotics tetracycline (tet"), kanamycin (kan®), streptomycin (sm®), sulfonamide (suf), and ampicillin (ampR) and to mercury (hg®) are shown. The resistant- determinant segment can move as a cluster of resistance genes. Tn3 is within Tn4. Each transposon can be transferred independently. [Data from S. N. Cohen and J. A. Shapiro, "Transposable Genetic Elements." Copyright 1980 by Scientific American, Inc. All rights reserved.] Tn10 IS10
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