For the bacteria circled on the left in the picture above, which describes the Gram status, shape, and arrangement? Gram negative, coccobacillus, tetrad Gram negative, cocci, tetrad Gram negative, tetrad, cocci Gram positive, cocci, tetrad
Q: Scopically after the Gram reaction Ammonium oxalate Crystal Gram's iodine Ethyl Alcohol Safranin…
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Q: For the bacteria circled on the left in the picture above, which describes the Gram status, shape,…
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Bacterial Morphology
The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology.
Bacterial cell structure
Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.
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- I U Cardo 12 1三 ニ 三 rI TI3 4. 111 I. 5. What are the basic shapes of bacterial cells? What are the basic Know the terms. Which types of organisms have axial filaments? How would classify the following in terms of gram stain reaction and shape and arrangement of the cells Escherichia coli? Staphylococcus aureus? Streptococcus pyogenes? Bacillus anthracis? Do these organisms cause any important diseases? arrangements? you IIIKousei works at a company that produces sweetened condensed milk. If one or more samples exceed 104 bacterial CFU/mL (or CFU/g) the entire lot produced will be rejected. Kousei's boss, Kaori, wanted to know if the lot passed the FDA guidelines. The following bacterial counts were obtained after serial dilution and pour plating of the samples: Type of Dilutions plated 10-4 10-³ 10'5 Colony Plate 1 Plate 2 Plate 1 Plate 2 Plate 3 Plate 1 Plate 2 Plate 3 Plate 3 244 Bacteria 288 251 99 81 75 24 21 15 Based on the bacterial counts obtained, will the lot be rejected? Yes, because the computed CFU/mL is 2.5 x 10^7 OYes, because the computed CFU/mL is 3.0 x 10^6 No, because the computed CFU/mL is 3.0 x 10^3 No, because the computed CFU/mL is 2.5 x 10^3 OYes, because the computed CFU/mL is 3.8 x 10^5 O No, because the computed CFU/mL is 3.0 x 10^3 O Information is lacking so CFU/mL cannot be computedA class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/L
- | 13 1 14 15 1 16. 17 1 18 1 What color would the bacterial cells have when viewed microscopically after the following treatment? Gram reaction Ammonium Gram's iodine Ethyl Alcohol Safranin oxalate Crystal violet Gram positive Gram negativeEscherichia coli Mycobacterium phlei Bacillus Micrococcus subtilis luteus A B Figure 1: Agar Slant Cultures of Bacteria (Gary E. Kaiser, Ph.D.- Professor of Microbiology) . Observe and describe the colour of the slant cultures (A-D) in fig 1. ( . Define the following terms: pure culture, sterile medium, inoculum, aseptic technique, and colony. What is the name of the cultures that you used . Where they gram negative or positive Define the following terms: psychrophile, mesophile, thermophile, and hyperthermophile.Answer the following questions: 1. Define a bacterial colony. 2. What is the difference between macroscopic and microscopic appearance of bacteria? 3. State the three standard terms used to describe single colonies on agar plates. 4. State and define the three types of growth that may be seen in a broth culture. 5. State three basic shapes of bacteria. 6. State and describe the different arrangements of cocci. 7. What is the difference between true motility and Brownian motion?
- The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution -5 Plate 3 10 dilution Plate 4 10 dilution Plate 5 107 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* TMTC* TMTC* 867 154 18The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution -3 Plate 1 10 dilution Plate 2 10-4 dilution Plate 3 10-5 dilution Plate 4 10 dilution Plate 5 10 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 1,957 65 21 1 0 mI completed the chart, and I'm supposed to figure out the unknown bacteria, there are 12 unknown culture, out of which of them resemble my chart the most? (My chart is the one in pink with the information filled out)
- The colony forming units (CFU)/ml in sauerkraut brine are given for different types of bacteria except for leuconostoc (shaded column). To obtain the Leuconostoc counts for different days, 40 microlitre of sauerkraut brine was 10-fold diluted and the CFU was determined by plating different dilutions on agar plates. For Day 0 56 CFU were counted in 10-1 dilution, Day 1 81 CFU were counted in 10-3 dilution, Day 2 86 CFU were counted in 10-3 dilution Day 5 78 CFU were counted in 10-5 dilution Calculate the CFU/ml of leuconostoc in the brine for the 4 different days. Show your calculationThis is how a hanging-drop slide is prepared. Figure from Macedo, Wikimedia Commons, 2016. 2 3 4 slide cover slip vaseline concavity drop of microbiological culture CA inoculation loop 11. Which slide gives you more information, the hanging drop or the stained slide? Why do you say so? 12. Why might it be valuable to know whether a bacterium is motile?izzes/00071/take/questions/600381 Regarding Gram staining, what will be the appearance of the Gram-positive bacteria if... All steps are done correctly? The slide is not heat-fixed prior to staining? Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? I Not enough acetone:alcohol was used? Carbol fuschin or safranin was omitted? W [Choose ] [Choose ] Purple Clear Pink Orange [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] 88 22°C