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- A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/LA laboratory technician performed a series of 10 fold serial dilutions shown below. he plated 0.1 mL from each dilution tube onto NA and got the follow numbers per plate dilution 1:10 1:10 1:100 1:1000 1:10000 1:100000 colony count >1000 >1000 808 303 38 3 calculate the concentration of bacteria in the undiluted stock (in CFU/mL)What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
- Answer the following questions What is the purpose of the 95% ethanol step in the Gram staining procedure? (1 Mark) xiii) What is the function of the following reagents used in Gram stain: a) Crystal Violet b) Gram’s iodine c) Acetone/alcohol d) Safranin IGram straining procedure. Indicate the following information A. Shape........ B. Stained color under microscope........ C. PG Wall thick, thin or both.....Decolorization in Gram-staining removes safranin from gram-negative cells True False
- Out of all these tests, which 2 would help further understand this unknown lab. (Gram Stain and Growth Result is already done) Possible Tests (pick 2 that would help know the unknown bacteria) Gram stain morphology & arrangement color and growth characteristics on agar 37C, 30C and 25C (room temp) CAMP test starch hydrolysis test bacitracin susceptibility test glucose, lactose, sucrose, maltose fermentation tests methyl red/MR test Mannitol MacConkey Phenol Red Vogues-Proskauer/VP test citrate utilization test urea hydrolysis test phenylalanine deaminase test SIM test (Sulfide/Indole/Motility) Nitrate reduction test Oxidase test Catalase testCould you spread plate in the usual Petri dish a 10mL inoculum?A cell is 25 μm wide when viewed at 1,000× magnification. Thismeasurement can also be written properly asa. 25 mm. c. 0.025 mm.b. 25,000 mm. d. 2.5 mm.
- TRUE or FALSE only! 1. Pure cultures contain only a single species and no other organisms. 2. Pour plate method is done by pipetting liquified warm agar into a petri dish containing an inoculum. 3. Flaming the test tube’s mouth is necessary so that the moisture that accumulated will evaporate. 4. After Gram staining, the Gram positive bacteria will appear blue while the Gram negative bacteria will appear red.List chemical agents that are effective at disinfecting smaller surfaces? Question 15 options: hydrogen perioxide steam Ultraviolet (UV) light Copper and/or silver coated surfaces All of the above Steam and UV light only2.1The number of bacteria in the samples need to be quantified. The laboratory uses a specificprotocol where the dilution factor on the spread plates must be:10-6; 10-7; 10-8Describe how you would prepare the required dilution series using the least amount of dilutionwater. 2.2 Calculate the amount of bacteria in the sample from the following results (show the steps in the calculation): Dilution factor Count10-6 27110-7 3510-8 21