#Genotype of strain1lacI + lacPlacO + lacZ + lacY+2lac lacPlacO + lacZ + lacY+3lacI+ lacP + laco C lacZ + lacY+4 lac lacPlacO + lacZ + lacy105lac lacP lacO + lacZ + lacY+lacl lacPlacO + lacZ-lacY+/6laclacPlacO + lacZ + lacY-Lactose absent Lactose absentLactose presentLactose presentPermeaseRogalPermease7810lac lacp+ lacO © lacZ + lacY+/lacl lacPlacO + lacZ¯lacY-lac lacP + lacO + lacZ + lacY¯/lacI+ lacP-lacO+ lacZ-lacY+lacı+ lacP-lacO© lacZ¯lacY+/lack lacP+ lacO + lacZ + lacY-lacl lacPlacO + lacZ + lacY+/lacI+ lacP + lacO + lacZ + lacY+ For the Escherichia coli lactose operon genotypes listed, + indicates a functional component, - indicates a nonfunctionalcomponent, superscript C indicates a constitutively active component, and superscript S indicates a component that isunresponsive to an inducer. Genotypes written on two lines and separated by a slash represent the bacterial chromosome and anadditional circular DNA strand, called a plasmid.For each E. coli strain shown on the table, use a plus sign (+) to indicate the synthesis of ẞ-galactosidase (ẞ-gal) and permeaseand a minus sign (—) to indicate no synthesis of these proteins. No glucose is present.+Answer Bank

Permit me to elaborate on these lac operon strains' genotypes and phenotypes: 1. lacZ+ lacZ+ lacY+…

Answered: # Genotype of strain 1 lacI + lacPlacO…

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the highest beta-galactosidase enzyme activity, when grown in the lactose medium. CAP It P+ O`Z+ CAP+ I P+ O+ Zt CAP+ It P- O+ Z+ CAP+ It P+ 0+ Z
A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the highest beta-galactosidase enzyme activity, when grown in the lactose medium. O CAP+ r* p* o* z O CAP* I P* o* z* O CAP* r* P O* z* O CAP I P* O z*
A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the lowest beta-galactosidase enzyme activity, when grown in the lactose medium. OF POZY Ort Ptot Z¹ Yt Ort p²o+z¹Y+ Orpt ot zty
For the given genotypes (associated with the lac operon in E. coli), indicate with a "+" or "-" whether functional B - galactosidase would be expected to be produced in the indicated conditions, assuming low glucose levels. B - galactosidase production Genotype No lactose With lactose a) I- O+ Z+ / F' I+ O+ Z+ b) IS O+ Z+/ F' I+ O+ Z+
Given the following genotypes, explain, by answering the questions in each number, how the mutation (identified by a (-) superscript) will affect E. coli grown in lactose medium. Will there be a complete set ofgene products? (Yes/No) Will the lac operon be turnedon/off? Will the cell survive? (Yes/No) a. i + p + o + z - y + b. i + p - o + z + y + c. i + p + o - z + y +
The genotypes of five merozygote bacteria are shown below. In each set, the genotype of the lac operon on the chromosome is on the left side of the slash (/) and the genotype of the lac operon on the F' plasmid is on the right side of the slash. Which of the merozygote bacteria listed below would be able to make high quantities of B-galactosidase in the absence of lactose? Mark all the answers that are correct. OIP*o*z* y*A*/F'I P oʻz*Y*A*. OI P o°z-Y*A*/F'IS P O*z*Y*A* nd of B * P*o*zY A /F'I P*o z*Y*A* erase e D I' P*o*z YA /F'I P*O z*Y* A* strand a NA poly should be 1S P*0°ZYA/FI P O*z*Y*A*
The following table lists 4 bacterial strains that are partial diploids for lac operon genes. Given the activity of beta-galactosidase measured for each strain in the absence (-lac) or presence (+lac) of lactose, complete the table by choosing the appropriate symbol (+, -, C, S) to indicate the allele of the gene or site missing from the table (blue numbers). + = wildtype, - = null mutation, c = constitutive, s =super repressor chromosome plasmid B-gal act. strain 10 Z A 1 C B 3 4 + C + 6 D 9 + 1 [Select] 3 [Select] 5 [Select] 7 [ Select] 9 [Select] | 0 2 + + 5 + 7 10 Z + -lac +lac 0.062 0.058 0.003 0.004 0.062 0.117 0.003 0.060 + 8 + 2 [Select] 4 [Select] 6 [Select] 8 [Select] 10 [Select]
Which of the following lac operon genotypes would allow for functional versions of all the structural enzymes of the lac operon to be expressed constitutively even in the absence of lactose? Group of answer choices I+ O+ Z+ Y+ A+ I- O+ Z- Y- A- I+ OC Z+ Y+ A+ IS O+ Z+ Y+ A+ I+ O+ Z- Y+ A+
A strain of E. coli has the genotypes shown below at the lac operon, where I = regulator gene, P = promoter, O = operator, Z = βgalactosidase gene, and Y = permease gene. The superscript+ indicates a wild-type allele,c indicates a constitutive mutation, and− indicates a defective mutation. For each genotype, indicate whether the enzyme will be synthesized or not synthesized when lactose is present or absent by placing a + for synthesis occurring and a − for synthesis not occurring in the appropriate blank. Explain your reasoning for each answer.
The genotypes of five merozygote bacteria are shown below. In each set, the genotype of the lac operon on the chromosome is on the left side of the slash () and the genotype of the lac operon on the F' plasmid is on the right side of the slash. Which of the merozygote bacteria listed below would be able to make high quantities of B-galactosidase in the absence of lactose? Mark all the answers that are correct. O i p*o°z¯ y*A*/F'I$ p*oʻz*y*A* I* p* o*Z¯YA*/F'T P*O*z*y* A* I* P* o*z*Y* A* /F'T P*O*z*y* A* O 1* p*o*z° YA*/F'T P*o°z*y*A* 1S p*0°2¯Y¯AZET P*O*Z*Y*A*
For the following genotypes of E. coli strains, state whetherenzyme activity is inducible (I), constitutive (C), or uninducible (U).Key to symbols in table:z = structural gene for  -galactosidasey = structural gene for permeasea = structural gene for transacetylasep = promoter (for lac operon)i = structural gene for the lac repressor (regulator gene)o = operator (binding site for repressor)Genotype  -gal (z) Permease (y) Transacetylase (a)i+ p+ oC z- y- a+i- p+ o+ z+ y- a- /i+ p- oc z- y+ a-i- p+ o+ z+ y- a+ /i+ p+ oc z- y+ a-i+ p+ o+ z- y- a+i- p- o+ z+ y- a+ /i- p+ o+ z- y+ a-iS p+ o+ z- y- a+i- p+ o+ z+ y- a+ /i+ p- oc z- y+ a-is p+ o+ z- y+ a+ /i+ p+ oc z+ y- a-i- p- o+ z+ y- a+ /i- p+ o+ z- y- a-is p+ oc z+ y- a+i+ p+ oc z+ y- a+
The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E,coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/). The possible answers indicate with a ʺ+ʺ or a ʺ-ʺ whether β-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in the presence of lactose. (Assume that glucose is not present in the medium.)Genotype F: I+ Oc Z-/ Fʹ I- O+ Z+ KEY:I+ = wild-type repressorI- = mutant repressor (unable to bind to the operator)Is = mutant repressor (insensitive to lactose)O+ = wild-type operatorOc = constitutive operator (insensitive to repressor)

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