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For the first modified gene sample, they observe that the intron is no longer being spliced out. Which of the lettered arrowheads indicates a location where this insertion could be? (There could be one answer or several. Give all answers that apply)

For the first modified gene sample, they observe that the intron is no longer being spliced out. Which of the lettered arrowheads indicates a location where this insertion could be? (There could be one answer or several. Give all answers that apply)

Human Heredity: Principles and Issues (MindTap Course List)
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter9: Gene Expression And Gene Regulation
Section: Chapter Questions
Problem 8QP: The following segment of DNA codes for a protein. The uppercase letters represent exons. The...
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Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small
number of base pairs randomly somewhere into the gene. They isolate several versions (with different
insertions) of this modified gene and carry out an RT-PCR using a primer that recognizes the
transcriptional start site area (eg. from +1 to +20), and a primer that binds to the polyA tail.
For the first modified gene sample, they observe that the intron is no longer being spliced out.
Which of the lettered arrowheads indicates a location where this insertion could be? (There
could be one answer or several. Give all answers that apply)

Here is a human gene, called cxx2. The following locations are marked: where transcription starts and ends, the start codon and the stop codon, and the boundaries of the two exons and the one intron. The open arrowheads (triangles) indicate specific locations in the gene. Use these locations (1-10) to answer the following questions. 1 2 transcriptional start site +1 3 Ĵ ATG exon/intron intron/exon boundary boundary intron transcriptional stop site 9 8 TAA 10
Transcribed Image Text:Here is a human gene, called cxx2. The following locations are marked: where transcription starts and ends, the start codon and the stop codon, and the boundaries of the two exons and the one intron. The open arrowheads (triangles) indicate specific locations in the gene. Use these locations (1-10) to answer the following questions. 1 2 transcriptional start site +1 3 Ĵ ATG exon/intron intron/exon boundary boundary intron transcriptional stop site 9 8 TAA 10
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