for the virulence of a phage. A bacteriophage solution of a given concentration is inoculated with the bacterial colonies and the number of plaques on the bacterial plate is counted. The plaques are the proxy for the number of phages present in the solution as each plaque is likely to come from a single parent phage. 3. Why is it that only a lytic phage will yield accurate results from this type of experiment? Why can't you use a latent or filamentous phage?

for the virulence of a phage. A bacteriophage solution of a given concentration is inoculated with the bacterial colonies and the number of plaques on the bacterial plate is counted. The plaques are the proxy for the number of phages present in the solution as each plaque is likely to come from a single parent phage. 3. Why is it that only a lytic phage will yield accurate results from this type of experiment? Why can't you use a latent or filamentous phage?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter26: Prokaryotes: Bacteria And Archaea

Section: Chapter Questions

Problem 11TYK

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An ade+ arg+ cys+ his+ leu+ pro+ bacterial strain is knownto be lysogenic for a newly discovered phage, but the siteof the prophage is not known. The bacterial map isleucysarghisadeproThe lysogenic strain is used as a source of the phage, andthe phages are added to a bacterial strain of genotypeade- arg- cys- his- leu- pro-. After a short incubation,samples of these bacteria are plated on six differentmedia, with the supplementations indicated in thefollowing table. The table also shows whether colonieswere observed on the various media.PresenceMedium Ade Arg Cys His Leu Pro of colonies1 - + + + + + N2 + - + + + + N3 + + - + + + C4 + + + - + + N5 + + + + - + C6 + + + + + - NNutrient supplementation in medium(In this table, a plus sign indicates the presence of anutrient supplement, a minus sign indicates that asupplement is not present, N indicates no colonies, and Cindicates colonies present.)a. What genetic process is at work here?b. What is the approximate locus of the prophage?
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