From the data in Table 2.5, compute the concentration of the undiluted and diluted ovalbumin samples (make sure to account for the appropriate dilution factors, DF, in the computation).

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter1: Introduction
Section: Chapter Questions
Problem 1.10QAP
icon
Related questions
Question

From the data in Table 2.5, compute the concentration of the undiluted and diluted ovalbumin samples (make sure to account for the appropriate dilution factors, DF, in the computation).

Table 2.5. Absorbance readings of the BSA standard solutions and crude ovalbumin samples.
Concentration of BSA standards, mg/mL
Absorbance at 595 nm
amstimetea
Undiluted ovalbumin sample
Diluted ovalbumin sample (1 in 7 dilution)
0.075
0.139
0.25
0.5
0.244
1.0
0.486
1.5
0.699
2.0
0.955
1.698
0.357
Diluted ovalbumin sample (1 in 10 dilution)
0.261
Transcribed Image Text:Table 2.5. Absorbance readings of the BSA standard solutions and crude ovalbumin samples. Concentration of BSA standards, mg/mL Absorbance at 595 nm amstimetea Undiluted ovalbumin sample Diluted ovalbumin sample (1 in 7 dilution) 0.075 0.139 0.25 0.5 0.244 1.0 0.486 1.5 0.699 2.0 0.955 1.698 0.357 Diluted ovalbumin sample (1 in 10 dilution) 0.261
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps with 2 images

Blurred answer
Knowledge Booster
Tools in Analytical Chemistry
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Fundamentals Of Analytical Chemistry
Fundamentals Of Analytical Chemistry
Chemistry
ISBN:
9781285640686
Author:
Skoog
Publisher:
Cengage
Appl Of Ms Excel In Analytical Chemistry
Appl Of Ms Excel In Analytical Chemistry
Chemistry
ISBN:
9781285686691
Author:
Crouch
Publisher:
Cengage
Macroscale and Microscale Organic Experiments
Macroscale and Microscale Organic Experiments
Chemistry
ISBN:
9781305577190
Author:
Kenneth L. Williamson, Katherine M. Masters
Publisher:
Brooks Cole