From your Lineweaver-Burk plot,the vlaues are:   Km Vmax Uninhibited 0.09 mmol/L 3.02 min/mmol Inhibited 6.22 mmol/L 9.98 min/mmol   By describing the potential changes in the kinetic parameters, identify and justify the type of inhibitor that was inhibiting the acid phosphatase activity.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter24: Lipid Biosynthesis
Section: Chapter Questions
Problem 23P
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From your Lineweaver-Burk plot,the vlaues are:

 

Km

Vmax

Uninhibited

0.09 mmol/L

3.02 min/mmol

Inhibited

6.22 mmol/L

9.98 min/mmol

 

By describing the potential changes in the kinetic parameters, identify and justify the type of inhibitor that was inhibiting the acid phosphatase activity. 

1/ acid phosphate activity (min/nmol)
-18
Lineweaver-Burk plot for acid phosphatase activity in the presence and in the absence of phosphate.
-13
-8
-3
17
12
7
2
-3
-8
2
7
acid phosphate activity = 0.6323 x p-nitrophenylphosphate + 0.1017
presence of phosphate ion
acid phosphate activity = 0.0318 x p-nitrophenylphosphate + 0.3312
absence of phosphate ion
1/ p-nitrophenolphosphate (L/mmol)
I
12
17
22
27
Transcribed Image Text:1/ acid phosphate activity (min/nmol) -18 Lineweaver-Burk plot for acid phosphatase activity in the presence and in the absence of phosphate. -13 -8 -3 17 12 7 2 -3 -8 2 7 acid phosphate activity = 0.6323 x p-nitrophenylphosphate + 0.1017 presence of phosphate ion acid phosphate activity = 0.0318 x p-nitrophenylphosphate + 0.3312 absence of phosphate ion 1/ p-nitrophenolphosphate (L/mmol) I 12 17 22 27
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