-gene--- 5'-GTCGACAAGCTTAAGCCTAAGCTTTAGCGAAGCTTGTCGAC-3' Recognition sites Sall 5'-GTCGAC-3' 3'-CAGCTG-5' Hindlll 5'-AAGCTT-3' 3'-TTCGAA-5'

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter15: From Dna To Protein
Section: Chapter Questions
Problem 10TYK: A part of an mRNA molecule with the sequence 5-UGC GCA-3 is being translated by a ribosome. The...
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A DNA sequence is shown below, which includes a gene as marked. You have the restriction enzymes SalI and HindIII available to you to excise the gene prior to its incorporation into a plasmid vector. Which would you use to excise the gene?

-gene-
5'-GTCGACAAGCTTAAGCCTAAGCTTTAGCGAAGCTTGTCGAC-3'
Recognition sites
Sall
5'-GTCGAC-3'
w w
3'-CAGCTG-5'
Hindlll
5'-AAGCTT-3'
3'-TTCGAA-5'
Transcribed Image Text:-gene- 5'-GTCGACAAGCTTAAGCCTAAGCTTTAGCGAAGCTTGTCGAC-3' Recognition sites Sall 5'-GTCGAC-3' w w 3'-CAGCTG-5' Hindlll 5'-AAGCTT-3' 3'-TTCGAA-5'
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