Given that the extracellular concentration of Cl- is approximately 120 mM, what is the intracellular concentration if the Nernst potential for Cl- is 39 mV. (T=298 K, R=1.987 cal/K·mol, F=23,062 cal/mol·V)
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- Using the Goldman-Hodgkin-Katz equation, determine what happens to the resting membrane potential if the ECF K+ concentration doubles to 10 mM.One of the important uses of the Nernst equation is in describing the flow of ions across plasma membranes. Ions move under the influence of two forces: the concentration gradient (given in electrical units by the Nernst equation) and the electrical gradient (given by the membrane voltage). This is summarized by Ohms law: Ix=Gx(VmEx) which describes the movement of ion x across the membrane. I is the current in amperes (A); G is the conductance, a measure of the permeability of x, in Siemens (S), which is I/V;Vm is the membrane voltage; and Ex is the equilibrium potential of ion x. Not only does this equation tell how large the current is, but it also tells what direction the current is flowing. By convention, a negative value of the current represents either a positive ion entering the cell or a negative ion leaving the cell. The opposite is true of a positive value of the current. a. Using the following information, calculate the magnitude of Na [ Na+ ]0=145mM,[ Na+ ]i=15mM,Gna+=1nS,Vm=70mV b. Is Na+ entering or leaving the cell? c. Is Na+ moving with or against the concentration gradient? Is it moving with or against the electrical gradient?What would be the free-energy change generated by the import of one mole of Na+ from a concentration of 10 mM to 150 mM with a membrane potential of −25 mV at 37°C? Give your answer without units and to one decimal place. F = 96.5 kJ/V•mol
- Calculate the effective quantity (g) of sodium chloride related to tonicity in 100 mL of an intravenous fluid labeled "5% dextrose in 0.45% sodium chloride," and indicate whether the solution is isotonic, hypotonic, or hypertonic.Calculate the following concentration given thisV=62 log 10 (C0/Ci ) for a positive ion at 37 degrees Celsius. What is theoretical ratio of solution ion across the membrane when the resting membrane potential is 124 mV?
- Calculate the equilibrium membrane potentials to be expected across a membrane at 37 ∘C, with a NaCl concentration of 0.50 M on the "right side" and 0.08 M on the "left side", given the following conditions. In each case, state which side is (+) and which is (−). Membrane equally permeable to both ions.Gout is caused by an error in metabolism that leads to abuildup of uric acid in body fluids, which is deposited as slightlysoluble sodium urate (C₅H₃N₄O₃Na) in the joints. If the extra-cellular [Na⁺] is 0.15 Mand the solubility of sodium urate is0.085 g/100. mL, what is the minimum urate ion concentration(abbreviated [Ur⁻]) that will cause a deposit of sodium urate?The difference in chemical potential of a particular substance between two regions of a system is −8.3 kJ mol−1. By how much does the Gibbs energy change when 0.15 mmol of that substance is transferred from one region to the other?
- Does 30% NaCl (MW=58.44g/mol,ionic) have the same, increased, or decreased osmotic pressure as 30% sodium phosphate? Why?Describe Bohr effect and give its physiological significance.Calculate the equilibrium membrane potentials to be expected across a membrane at 37 ∘C, with a NaCl concentration of 0.50M on the "right side" and 0.08 M on the "left side", given the following conditions. In each case, state which side is (+) and which is (−). Membrane permeable only to Cl−.