Given the data below, plot the titration curve of the unknown amino acid. Determine the isoelectric point and the identity of the unknown amino acid. Volume of 0.1M KOH(mL) pH 0 1.45 0.5 1.43 1 1.45 1.5 1.47 2 1.49 2.5 1.51 3 1.54 3.5 1.58 4 1.62 4.5 1.65 5 1.7 5.5 1.74 6 1.8 6.5 1.86 7 1.92 7.5 2 8 2.09 8.5 2.22 9 2.36 9.5 2.57 10 2.84 10.5 3.42 11 5.44 11.5 8.4 12 9.72 12.5 10.41 13 11.21 13.5 11.84 14 12.19 14.5 12.37 15 12.51 15.5 12.63 16 12.71 16.5 12.78 17 12.83 17.5 12.89 18 12.94 18.5 12.99

Appl Of Ms Excel In Analytical Chemistry
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ISBN:9781285686691
Author:Crouch
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Chapter8: Polyfunctional Acids And Bases
Section: Chapter Questions
Problem 14P
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  1. Given the data below, plot the titration curve of the unknown amino acid. Determine the isoelectric point and the identity of the unknown amino acid.

    Volume of 0.1M KOH(mL)

    pH

    0

    1.45

    0.5

    1.43

    1

    1.45

    1.5

    1.47

    2

    1.49

    2.5

    1.51

    3

    1.54

    3.5

    1.58

    4

    1.62

    4.5

    1.65

    5

    1.7

    5.5

    1.74

    6

    1.8

    6.5

    1.86

    7

    1.92

    7.5

    2

    8

    2.09

    8.5

    2.22

    9

    2.36

    9.5

    2.57

    10

    2.84

    10.5

    3.42

    11

    5.44

    11.5

    8.4

    12

    9.72

    12.5

    10.41

    13

    11.21

    13.5

    11.84

    14

    12.19

    14.5

    12.37

    15

    12.51

    15.5

    12.63

    16

    12.71

    16.5

    12.78

    17

    12.83

    17.5

    12.89

    18

    12.94

    18.5

    12.99

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