GMO modified food lab: TOP Tier: Lane 1: ladder (20ul), Lane 2: positive control, Lane 3: Bell pepper, Lane 4: Apple, Lane 5: Corn chip, lane 6: carrot, Lane 7: Almond, Lane 8: Corn, lane9: blank lane, Lane 10: positive control, Lane 11: Ladder ( 15 ul). Bottom Tier: Lane 1: ladder, Lane 2: Positive control, Lane 3: Radish, Lane 4: Radish, Lane 5: Strawberry, Lane 6: Sweet Potato, Lane 7: Nuts, Lane 8: Apple, Lane 9: Blank, Lane 10: Blank Lane 11: Ladder (10 u) , Lane 12: Ladder ( 15 ul). Three primers are used: Chloroplast gene segment (500bp), 2) CaMV 35S promoter (200bp), 3) Bacillus thuringiensis (BT/cry1f 125bp). 1- Summarize your finding for each lane. Discuss the importance of ladder/marker and control. Your take about what is GMO and what is not. 2- Your input after analyzing the data- special comments on primer dimer (apple and corn), why is the chloroplast gene shows double bands in kale and carrot (some isoforms of that gene 3- Do you think your PCR worked? Why did you use control?
GMO modified food lab:
TOP Tier: Lane 1: ladder (20ul), Lane 2: positive control, Lane 3: Bell pepper, Lane 4: Apple, Lane 5: Corn chip, lane 6: carrot, Lane 7: Almond, Lane 8: Corn, lane9: blank lane, Lane 10: positive control, Lane 11: Ladder ( 15 ul).
Bottom Tier: Lane 1: ladder, Lane 2: Positive control, Lane 3: Radish, Lane 4: Radish, Lane 5: Strawberry, Lane 6: Sweet Potato, Lane 7: Nuts, Lane 8: Apple, Lane 9: Blank, Lane 10: Blank
Lane 11: Ladder (10 u) , Lane 12: Ladder ( 15 ul).
Three primers are used: Chloroplast gene segment (500bp), 2) CaMV 35S promoter (200bp), 3) Bacillus thuringiensis (BT/cry1f 125bp).
1- Summarize your finding for each lane. Discuss the importance of ladder/marker and control. Your take about what is GMO and what is not.
2- Your input after analyzing the data- special comments on primer dimer (apple and corn), why is the chloroplast gene shows double bands in kale and carrot (some isoforms of that gene
3- Do you think your PCR worked? Why did you use control?
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