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Biomedical Instrumentation Systems

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter19: Biomedical Laboratory Instrumentation

Section: Chapter Questions

Problem 4Q

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Question

Percentage of bacteria surviving
100
80
60
40
20
10
0
1% iodine in 70% ethanol (tincture of iodine)
20
B) Zephiran 1:1000
40
C) Tincture of Zephiran
Soap and water
Aqueous Zephiran 1:1000
D) 1% iodine in 70% ethanol
Tincture of Zephiran
▪▪▪▪▪▪▪▪▪▪▪▪▪▪▪▪
60
80
Time (sec)
You are testing the efficacy of different treatments on eliminating SARS-Cov-2 from surfaces. Which treatment is the least effective?
A) Soap and water
100
120

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TSI Test plz answer all questions Why does TSI contain less glucose than sucrose or lactose? Does an acidic slant indicate that the microbe is a coliform? Explain your answer. What is the function of iron in this medium? Production of H2S by bacteria growing anaerobically in the intestines is common. Some H2S is absorbed into the circulation and some is expelled as intestinal gas. Less commonly, in certain individuals, H2S producing bacteria can live in the plaque built up around their teeth. What undesirable effect might that lead to? conclusion
The number of bacteria in saliva samples was determined by collecting the saliva, making serial dilutions, and inoculating nutrient agar by the pour plate method. The plates were inoculated aerobically for 48 hours at 37°C. What can you conclude from this data? Why? Mouthwash CFU per ml in salvia Before Use After Use Listerine 2.22 x 107 1.22x107 Schmidts Mouthwash 2.42 x 107 3.22x105 Biotene Mouthwash 3.21 x 107 2.12 x106
2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)
A student inoculates Escherichia coli into two test tubes containing the same sterile liquid medium, except the first tube also contains a drop of a chemical disinfectant with an antimicrobial effect. After 24 hours of incubation, the first tube remains clear while the second tube becomes cloudy with bacteria. How would you go about determining if the chemical is bacteriostatic or bactericidal?
One ml of a water sample was placed in a Petri dish with melted agar. After incubation, 129 colonies were counted. How many bacteria (CFU/ml) are in the sample? Thank you
I. Match the bacterium being described in column X with its best classification found in column Y. Choose the best answer. Column X1. Staphylococcus aureus can be grown on mannitol salt agar that contains 7.5% NaCl. The bacterium is________. 2. Lactobacillus acidophilus grows best at pH 4.5. It is considered a(n) ________. Column Y A. acidophile B. alkalophile C. barophile 3. A bacterium that thrives in the Great Salt Lake but not in fresh water is probably a ________.4. Bacteria isolated from the bottom of the ocean need high atmospheric pressures to survive. They are________.5. A bacterium that thrives in a soda lake where the average pH is 10.5 can be classified as a(n) ________. D. halotolerant E. halophile
EXPERIMENT : REMOVAL OF WATER FROM MICROBIAL CELLS USING FREEZE DRYING METHOD Objective To learn on the technique of freeze drying for microbial sample preparation and preservation. Result Weight of test tube (g) -Wi 15.34 Weight of test tube with cells (g) - Ww 34.65 Amount of water removal = weight of test tube with cells – weight of test tube = 34.65 g – 15.34 g = 21.16 g QUESTION: Discuss the result and explain the freeze drying method that used in this experiment.
You are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 9) What makes the bacteria Gram negative?10) Approximately how long would it take you to finish the entire procedure
Besides dehydrated foods, how might the amount of water available to microbes be decreased? Group of answer choices Treatment by non-ionizing radiation. Treatment at high pressures for extended periods of time. Storage at temperatures between -2 and 4 °C. Pasteurization Addition of solutes such as salt or sugar.
Identify which antibiotic was used per set-up (see figure). Describe the result in the graphs provided to help you explain your answer. Listed below are some essential information.Antibiotic A: 0.5 kDa protein, targets peptidoglycanAntibiotic B: 20 kDa protein, targets peptidoglycanAntibiotic C: Cationic antimicrobial peptideAntibiotic D: Targets lipopolysaccharideStaphylococcus aureus: gram-positive bacteriumVibrio cholera: gram-negative bacteriumMethanosarcina: an archaean bacteriumCationic antimicrobial peptides (CAMPs): these positively charged antibiotics are attracted to the negatively charged cell wall and membrane. They are hydrophobic, and they insert into the membranes to create pores.
Per the USDA, whole, unpasteurized fresh eggs can contain no more than 50,000 CFU/mL bacteria in a standard plate count. You are curious if the fresh eggs that you buy from your neighbor are considered safe to consume so you use your eScience Microbiology kit to test these eggs using direct plate count after serial dilution. After you complete the experiment, you obtain 74 countable colonies from the 10-2 dilution plate. The inoculum volume you plated was 0.1 mL. How many bacteria are present in 1 mL of the egg you sampled? Are these eggs considered safe to consume per USDA standards?
Original culture 50 colonies from .1mL, 1:10000 estimate the number of bacteria per unit volume in the original culture.

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