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- A patient exhibits a boil on his neck. You, as a microbiology technician, are asked to identify the causative organism and determinewhether it is pathogenic. Describe the procedure that you would follow tomake this determination.Below is a scenario requiring the use of laboratory equipment and instruments. A watery fecal sample is tested for the presence of Bacillus cereus and Vibrio cholerae. The sample is stored in a screw capped tube. You are tasked to grow the Bacillus in a solid medium while Vibrio in a liquid medium. Choices: A. Incubator, B Hanging Drop Slide , C.Petri Dish, D. Drying Oven, E. Bunsen Burner, F. Inoculating NeedlE , G. Volumetric Pipette, H. Screw-capped Tubes, I. Hot plate, J. Centrifuge, K. Inoculating Loop, L. Microscope, M. Beaker, N. Biosafety Cabinet 1. What could be used to determine if there are more bacteria in the sample aside from Bacillus and Vibrio? 2. What should you use if you want to observe the natural motility of Bacillus under a Brightfield microscope? 3. Where should you perform the task of sterilization and inoculation? 4.What should be used to transfer the sample to the broth? 5. What could be used to separate the solid from the liquid portion of the…Match the best selection media with the organism A. Carr B. MRS, Carr and WLD Positive C. MRS or WLD D. Lysine media a. Pediococcus damnosus b. Oenococcus oeni c. Candida vini d. Dekkera intermedium e. Lactobacillus brevis f. Acetobacter pasteurianus
- Which of the following tests can be used to distinguish between Enterobacter and Pseudomonas strains? blood agar EMB oxidase catalase ureaseIn the given below options which of the following is not one of the six “I’s”? a. inspection b. incubation c. identification d. inoculation e. illuminationWhich of the following is not an acid fast bacterium: A. Mycobacterium tuberculosis B. Salmonella enterica C. Mycobacterium leprae D. Nocardia asteroides
- Staphlococcus epidermis appears ............ whereas Escherichia coli appears ........ after the Gram stain. a)blue;red(pink) b)red (or pink);purple c)orange;red(or pink) d)red(or pink);blue e)orange purpleWhy don’t health departments routinely test for pathogens instead of using a sewage indicator?Which of the following tests can be used to distinguish between group D streptococcus and other streptococcus bacteria? Mannitol Salt agar Coagulase test McConkey agar Bile eschulin agar Simmon's Citrate agar
- Which of the following methods is most sensitive for identifyingdifferent strains of a microbe?a. microscopic examination b. hemolysis on blood agar c. DNA analysis d. agglutination testAny process that destroys the non-spore-forming contaminants oninanimate objects isa. antisepsis b. disinfection c. sterilization d. degermationSanitization is the freedom from viable forms of microorganisms. a. False b. True