How can you calculate the final concentrations of these when final volume is 50 µl? • 2 µl of 5 ng DNA template (α2 cDNA in pAWneo2 vector, stock concentration 2.5 ng/µl) • 1 µl of dNTP mix (10 mM dATP, 10 mM dTTP, 10 mM dCTP and 10 mM dGTP) • 2 µl of α2I forward primer (5 µM) • 2 µl of α2I reverse primer (5 µM) • 2.5 µl of OneTaq polymerase (0.5 U/µl)

How can you calculate the final concentrations of these when final volume is 50 µl? • 2 µl of 5 ng DNA template (α2 cDNA in pAWneo2 vector, stock concentration 2.5 ng/µl) • 1 µl of dNTP mix (10 mM dATP, 10 mM dTTP, 10 mM dCTP and 10 mM dGTP) • 2 µl of α2I forward primer (5 µM) • 2 µl of α2I reverse primer (5 µM) • 2.5 µl of OneTaq polymerase (0.5 U/µl)

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.3: Microhematocrit

Problem 3RQ

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