How does freeze fracture microscopy work and how has this technique improved membrane protein localization? our knowledge of

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter1: The Clinical Laboratory
Section1.10: The Microscope
Problem 11RQ
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I think it works by freezing the bilayer of the membrane and then hitting it sharply with a diamond knife. The sharp hit creates a fracture in the membrane and usually that fracture follows the plane between the two layers of the membrane lipid.

How does freeze fracture microscopy work and how has this technique improved our knowledge of
membrane protein localization?
Transcribed Image Text:How does freeze fracture microscopy work and how has this technique improved our knowledge of membrane protein localization?
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