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How much serum is needed to create 2μL of a 1:20 dilution?
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- Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μLIf you make a five-tube twofold dilution using 2 mL of serum, what is the concentration of serum in tube #4?What level of RF in serum is clinically significant? Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the qualitative test? Describe how the RF concentration is computed?