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How we can select Tm value for the primers? Please answer at your own words.
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- Why different temperatures are used in a PCR reaction? Please answer at your own words.What are the things to consider in making a good primer? Does temperature affects the primer design? Discuss briefly.What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis profile from the Sanger sequencing method?
- I believe the answer would be A and D, but I am not sure because the recombinant proteins are significant only in the last to lanes. As the first lane is an empty vector. I appreciate your help.What are the components require in a PCR reaction? And justify them? Please answer at your own words.Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the original DNA sample used for sequencing? GGTAACC CCAATGG GGTTACC CCATTGG