What do you mean by Southern Blotting technique?

Q: What is T DNA tag?

A: T DNA (Transferable deoxyribonucleic acid) tag is an important phenomenon in molecular biology and…

Q: How do Northern and Western blotting differ from Southern blotting?

A: The various blots are used to identify a specific target molecule present in a complex mixture. The…

Q: What are (ORFs?

A: Translation is the process of polymerization of amino acids to form poly peptide chain. The decoding…

Q: Define about Southern blotting ?

A: Introduction: Blotting refers to the transfer of the fractionated molecules from the gel to the…

Q: When we need to perform Southern, Western and Northern blotting? How they are different from one…

A: Blotting is the technique used to transfer the DNA, RNA, or protein sequence on a carrier membrane…

Q: What do the spacers within the CRISPR region correspond to?

A: CRISPR that is clustered regularly interspaced short palindromic repeats. It is found in the…

Q: Why was it necessary to soak the gel in hydroxide before performing the blot?

A: Gel electrophoresis is a technique which is used to separate the molecules based on the size of the…

Q: Restriction mapping of the delta chromosome I need help with question two please

A: the mean size is close to 4000 bp. DNA fragments of this length are useful in the lab, since…

Q: What does red list contains?

A: The international union for conservation of nature (IUCN) is an international organization that…

Q: Which statement is right the polyclonal antibody is expensive to produce the monoclonal antibody…

A: Antibodies are giant Y-shaped proteins known as immunoglobulins that are created by B cells as a…

Q: Why reverse transcriptase is used ?

A: Viruses can have either DNA or RNA as its genetic material. The DNA or RNA can further be single…

Q: What is Western blotting ?

A: Blotting refers to a technique by which a biomolecule is transferred from one medium to another…

Q: How do you use CRISPR to treat a patient with sickle cell anemia? Please provide a detailed…

A: In sickle cell anemia the blood cells become sickle shaped. This may result into painful blood flow,…

Q: How is western blotting important in biology?

A: In Western blotting (WB), target proteins are transferred to a hydrophobic membrane after SDS-PAGE…

Q: could you write about 4-5 sentences explaining what CRISPR is

A: Humans started manipulating genome initially by controlling breeding and through selection of…

Q: What is west Blotting

A: Blotting is a technique used in molecular biology to identify whether a given sample is DNA or RNA…

Q: Distinguish between a Northern, Southern and Western blot

A: Southern blotting is a blotting process for detecting particular DNA sequences, Northern blotting is…

Q: Where does the primer come from?

A: Naturally, Primers are single-stranded nucleic acid sequences that initiate the synthesis of DNA…

Q: Use the genetic decoder provided to assist in answering this question.\ Second letter A UUU UUC Phe…

A: Introduction: The process of polymerization of amino acid to form a polypeptide is called…

Q: what is the function of ubiquitination

A: Ans- Ubiquitin which is a small protein found everywhere in the body in all the tissues, helps to…

Q: what is the difference between western blot and sds page

A: A western blot is a laboratory method used to detect specific protein molecules from among a mixture…

Q: I need help defining what is DNA Chromatography.

A: Chromatography is known as the most efficient methods that is utilized in purifying as well as…

Q: How would you simply explain Protein Analysis (Western blotting)?

A: Proteins, also known as polypeptides, are made up of amino acids. They’re large, complex molecules…

Q: Why was it risky for the chinese scientist to change the twin’s genome sequence?

A: It was risky for the chinese scientist to change the twin's genome sequence as it may lead to more…

Q: Why is it necessary to boil the probe before you use it in your southern blot?

A: Southern blotting is a molecular biology technique that is used to detect a person's DNA. This…

Q: Please help me to have an outline for my presentation about Pesticide and Antibiotic Resistance…

A: Antibiotic resistance genes can be transmitted from cell to cell through recombination, conversion,…

Q: Describe the process of Southern blotting.

A: Introduction: Southern blotting works on the principle involving the separation of fragments of DNA…

Q: What are the main procedures involved in a western blot?

A: BASIC INFORMATION WESTERN BLOT This process is used to find some specific type of proteins which are…

Q: What is Spectral Karyotyping.?

A: In spectral karyotype the homologous pairs of chromosomes are manipulated in way that they have…

Q: Define the historical significance of Southern blotting ?

A: DNA profiling is the process of determining an individual's unique DNA pattern. DNA profiling can…

Q: What kind of vector is this?

A: This is the picture of human flea , Pulex irritans.  It is very poor vector.  It cause trench…

Q: Did Wat son and Crick steal Rosalind Franklin's data?

A: Intellectual theft and plagiarism are serious accusations in the academic world. Plagiarism is…

Q: Table I CAC GTA GACTGAGG ACTC CACGTA GACTGAGG ACAC Wild-type beta-globin gene fragment Sickle-cell…

A: Abrupt changes in the DNA sequence of nucleotides is called mutation. Even a single nucleotide…

Q: Briefly Explain different applications of western blotting. Please explain at your own words.

A: A western blot is an analytical molecular biology laboratory method that is used in the detection of…

Q: Where did western blot get its name?

A: The western blot (also known as the protein immunoblot) or western blotting is molecular biology and…

Q: Why do researchers believe they can use CRISPR/Cas totreat disease?

A: Introduction  CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats.  This is…

Q: Describe the steps in Southern blotting.

A: Southern blot is a technique used to analyse information about identity, size, and abundance of DNA.…

Q: what is triple code Your answer

A: Genetic code can be described as the ordering of nucleotides in DNA molecules which carries the…

Q: Does karyotyping requires dna extrecation?

A: Karyotyping is the process of pairing and ordering all the chromosomes of an organism. Karyotypes…

Q: What is Northern blot analysis ?

A: The nucleic acids transfer on a solid phase membrane for identification is called the blotting…

Q: Is a Primer written in the RNA or DNA form

A: Primers are short nucleotide sequence that is complementary to template strand and prime DNA for…

Q: a student uses a monoclonal antibody to detect a protein using a direct Western-blotting protocol…

A: Secondary antibodies are used to detect a target after a particular primary antibody has been bound…

Q: The method that Mandla should use to distinguish recombinants from non-recombinants is called th…

A: Recombinant DNA (rDNA) molecules are DNA molecules created by laboratory genetic recombination…

Q: In Northern and Western blotting, what is the purpose of gel electrophoresis?

A: Blots are strategies for moving DNA (deoxyribonucleic corrosive), RNA (ribonucleic) and proteins…

Q: What are the main steps of Western Blotting?

A: The technique of Western blotting is a well-known and commonly used method for detecting and…

Q: What is Ponceau S and why do we use it during western blotting?

A: Western Blotting : It is an analytical technique of molecular biology which is used to separate the…

Q: What does RFLP stand for

A: Molecular biology is a specialized branch of Biology and Biochemistry, which are specifically…

Q: Which of the following pieces of information about our recombinant DHFR protein do you predict to…

A: Western Blotting Technique is a common procedure for the detection and analysis of proteins. It is…