How would I figure out how many dilutions to make given cell density ? And volume to plate ? For example, I want to plate 0.1 ml and want 200 colonies how many dilutions would i need to make, given 7.5 x10^5 cells/ ml
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How would I figure out how many dilutions to make given cell density ? And volume to plate ? For example, I want to plate 0.1 ml and want 200 colonies how many dilutions would i need to make, given 7.5 x10^5 cells/ ml
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- If you added 5 mL of a 20X tocopherol to 5 mL of cell culture at a density of 2.4 x 107 cells/mL, what would the final concentration of tocepherol and cell density be?You have a culture of cells at 3.5 x 108 cells/ml and you want to prepare a fresh 5 ml culture with a starting density of 1 x 104 cells/ml. What volume of the old culture do you add to the new 5 ml culture?You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell density of this culture?
- The culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per mL. For your experiment, you first dilute the culture 100 fold. Assuming that there is no lab phase and that the cells remain in exponential growth the entire time, what is the cell density (cells/mL) after 10 hours?When the number of colony-forming units per milliliter is calculated, you will focus your counts on the Petri plates with __________.A 2x10-6 dilution of your bacterial culture yielded 10 colonies. What was the cell density (incells/ml) of your bacterial culture?
- You want to determine the amount of cells in a culture. You dilute the suspension to 10^-4 and plate 100ul onto an agar plate. After overnight incubation there are 30 colonies on your plate. How many cells are in your original suspension (assume your culture is 1 Liter)?FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?
- You use a spectrophometer and estimate the concentration of bacteria in a urine sample to be 4.6 x 10^5 cells/ml. Assuming you will plate 0.1ml of your samples, to what factor should you dilute your sample to get a countable number of colonies? Rearrange Original Cell Density formula to solve.How would you record your observation of a plate containing 305 colonies?A plate with 15 colonies?Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?