FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification?
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- FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification?
2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?
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- Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and dilution factors? What are 2 disadvantages?What is the difference between total magnification and objective magnification? You are looking at an organism on the 20x objective What is the total magnification? If the organism takes up 62 units on the ocular micrometer, how big is the organism is micrometers? Show your work.
- Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X oil immersion lens. Would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm?What are the processes of concentrate samples analysis in Uv-visible spectroscopy? Please shortly answer at your own words(3-4 lines).if 5X instead of 10X oculars were used with the same objectives now on your microscope, what magnifications would be achieved?
- Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the total magnification of the image under microscope? Show your solution.If 5X instead of 10X oculars were used with the same objectives now on your microscope, what magnifications would be achieved? Explain your answer.Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Questions: What is the total magnification of the image under microscope? Show your solution. What is the distance of one ocular division? Show your solution. What is approximate cell length of Cell A, Cell B, and Cell C? What is approximate combine cell length of Cell A and Cell B? Show your solution. Remember: Always measure the cell length on the longest middle section of the cell!
- In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.You used the stage micrometers to measure the FOV for your microscope at 400x total magnification at 225 µm Use the formula Mag1 × FOV1 = Mag2 × FOV2 to calculate the FOV for your microscope at 1000x total magnification___________ 40x total magnification____________ Convert the 40x FOV to mm: _____________when viewing an organism under the 40x objective lens, you estimate that 6 organims could fit across the DFV if they were laid end to end and 20 could fit if stacked side by side. What is the length and width of this organism in micra?
