If you find an RFLP in a gene when comparing sequences of two different people, does that mean that the gene is disrupted in one person? RFLP are never associated with a gene disruption Yes in the RFLP is in an exon It may be, or the RFLP may have no consequence on gene activity Yes, the gene must be disrupted in one of the people Yes, if the RFLP is in an intron
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- A molecule geneticist hopes to find a gene in human liver cells that codes for an important blood-clotting protein. He knows that the nucleotide sequence of a small part of the gene is GTGGACTGACA. Briefly explai n how to obtain the desired gene.Where in a gene would a 4-bp insertion mutation havethe least effect on gene expression?a. Intronsb. Exonsc. Regulatory regionsd. Introns and exonsA gene mutation changes an AT base pair to GC. This changecauses a gene to encode a truncated protein that is nonfunctional.An organism that carries this mutation cannot survive at high temperatures.Make a list of all the genetic terms that could be used todescribe this type of mutation.
- In nature, the vast majority of individuals within a species have unique genetic codes. In the last few decades, human geneticists have discovered ways to artificially duplicate a genetic code. Two organisms with identical genetic codes might be the result ofA. CloningB. MutationC. Coevolution. While perusing the E. coli K12 genome sequence,you come across a gene with no known function. Theamino acid sequence of the gene’s protein productshows weak similarities with known porins, proteinsthat cross a cellular membrane to let molecules suchas amino acid or sugar nutrients (or drugs like penicillin) pass through. Some porins are nonspecific and letany solute up to a certain size transit into the cell.Other porins are specific and allow the transit ofcertain sugars but not others. What genetic experiments could you do to try to determine whether thisnew gene has a specific function in allowing bacterialcells to scavenge the sugar maltose from the environment? Describe scenarios that might complicate yourexperimental approach.An alien visits Earth and is found to have the same 'genetic code' as humans. However, the alien has many more 'stop' codom which replace codoes that are redandant in the human genetic code. If nucleotide substitution errors occur at the same rate for aliens and humans, the aliens would have many more of whichkind of mutations?a.) missense mutation only b.) silent mutations only c.) nonsense mutations only d.) antisense mutations only e.) silent and missence mutations
- The amount of DNA inintronic regionof genes can be grwater than the amount of DNA in exons. trueResearchers produced transgenic corn that contained an extra codon for phenylalanine andhad a high oil content. Suppose an existing commercial variety of corn was modified in thesame way and sold to farmers. Would you consider this variety to be a genetically modifiedorganism ? Should the corn produced by this variety be labeled as agenetically modified food product?. You obtain the DNA sequence of a mutant of a 2-kb genein which you are interested and it shows base differencesat three positions, all in different codons. One is a silentchange, but the other two are missense changes (they encode new amino acids). How would you demonstratethat these changes are real mutations and not sequencing errors? (Assume that sequencing is about 99.9 percent accurate.)
- Since it was deciphered four decades ago, somehave claimed that the genetic code must be a frozen acci-dent, while others have argued that it was shaped by nat-ural selection. A striking feature of the genetic code is itsinherent resistance to the effects of mutation. For example,a change in the third position of a codon often specifies thesame amino acid or one with similar chemical properties.The natural code resists mutation more effectively (is lesssusceptible to error) than most other possible versions, asillustrated in Figure Q1–1. Only one in a million comput-er-generated “random” codes is more error-resistant thanthe natural genetic code. Does the extraordinary mutationresistance of the genetic code argue in favor of its origin asa frozen accident or as a result of natural selection? Explainyour reasoning.The human genome contains about 500 genes fortRNAs.a. Do you think that each one of these tRNA geneshas a different function?b. Can you explain why the human genomemight have evolved so as to house so manytRNA gen es?........ cen not be true about in ditu hybridization. Use to reveal where gene expressed at mrna lvel. perform in none live animal/ tissues. reveal whether 2 genes are active in same cell. determine the function of gene