If you have the same protein loaded in two different lanes of an SDS-PAGE stained with Coomasie blue, and one lane has a darker, thicker band of protein than the other lane, can you say that there is more protein in the lane with the darker band? Why or why not?

Q: This gel has approximately 10mg of protein in each lane.  * What would this gel look like if you…

A: A particular type of gel has a specific sensitivity, SDS-PAGE gels have a high sensitivity that 2ug…

Q: Are all the proteins separated properly using SDS-PAGE? Why or why not?

A: Proteins are massive biomolecules and macromolecules made up of one or more long chains of amino…

Q: Multisubunit proteins in which some or all of the subunitsare identical are referred to as…

A: Multisubunit proteins are considered as those proteins, which are composed of more than one protein.…

Q: When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands…

A: Zymography, also known as substrate gel electrophoresis, is an experimental technique for…

Q: Is the ubiquitination relatively stable or highly dynamic?  how does ubiquitination become detached…

A: The translation is a process through which ribosomes synthesizes polypeptide chains using mRNA. This…

Q: What is the difference between the two salt precipitation methods: salting in and salting out?…

A: The solubility of a protein in solution depends on the concentration of the salt present in…

Q: In an experiment of egg white and calamansi juice in protein denaturation, what will happen?

A: Proteins are one of the  most important metabolites in living organisms, which is almost present in…

Q: first 20 amino acids of the CFTR protein?

A: CFTR is Cystic Fibrosis transmembrane Regulator Protein is an important protein that maintains the…

Q: In TLC, why are the colors of the peaks for each amino acid are different depending on the types of…

A: TLC stands for Thin Layer Chromatography. It is a refined technique that is used for isolating or…

Q: Which is the most deleterious type of amino acid substitution? Explain.

A: Amino acid substitutions are due to mutations in the the DNA that codes for the mRNA.  These…

Q: How do 21st and 22nd aminos acids effect protein

A: Selenocysteine (Sec) and pyrrolysine (Pyl) are unique amino acids that are cotranslationally…

Q: If the DNA gene is TAC GGT TTA CAG, which amino acids will be put together?

A:

Q: This is a picture of an SDS-PAGE gel. In the left lane are protein standards with molecular masses…

A: SDS-PAGE is used determining the molecular weight (MW) of an unknown protein. The first step the…

Q: Which amino acid is classified as neutral and non-polar?

A: Amino acids when joined together by a peptide bond synthesizes the protein molecules. Amino acids…

Q: When a protein was  denatured and lost its function due to its spatial structures were disrupted.…

A: Denaturation includes the breaking of a considerable weak linkages, or bonds (e.g., hydrogen bonds),…

Q: fter processing all of your protein samples in the laboratory, you are left with one sample to…

A: The mechanical process that is used for the separation of particles in a solution includes…

Q: Lactose exists in two Why?

A: Disaccharides are made up of two monosaccharides. Lactose is a disaccharide made up of glucose and…

Q: Provide a brief definition for a polymorphic protein

A: Polymorphism means have many types of morphology and structure. It is a condition when more than one…

Q: What other approaches can be used to determine protein concentration?

A: Introduction :  Proteins are nitrogenous organic substances made up of one or more elongated chains…

Q: The change of one conformation of protein to another is called    a. allosteric transversion b.…

A: Option b Allosteric transition

Q: A certain protein is exposed to high levels of β- Mercaptoethanol (BME). Even after the BME is…

A: Proteins are biomolecules formed of amino acids. There are lipoproteins and glycoproteins that have…

Q: A gel filtration column with a fractionation range of 1.5-20 kDa is used to separate out the…

A: Gel filtration chromatography is a technique that is used to separate molecules based on their…

Q: The limit for G-200 beads is 5000-600,000. When you pass two proteins- Protein A (75, 000), Protein…

A: Proteins are amazing molecules that make everything simple inside the cell. Protein molecules are…

Q: Identify a disease or condition related to protein mis-folding.  Which protein is misfolded?  Is…

A: Accumulation of misfolded proteins cause disease various neurodegenerative diseases, such as amyloid…

Q: Proteoglycans are often made up of glycosaminoglycans (chondroitin sulfate, keratan sulfate, and…

A: Proteins are the basic building components of our bodies. They are big and complicated…

Q: What does the allostery of a protein mean?

A: The term "allostery" has been evolved from past decades. This term has been coined from the era of…

Q: Given enough time, will all denatured proteins spontaneously renature?

A: Proteins are building blocks of the body and they are composed of amino acids and linked via…

Q: Given the Ramachandran Plot below, identify the protein components that could adopt the phi-psi…

A: The bond between nitrogen and alpha carbon (N-Calpha) in amino acid is known as phi bond. On the…

Q: Select the amino acid sequence that would most likely be located in the interior of a water soluble…

A: Peptides are composed of a linear chain of amino acid sequences attached to each other via peptide…

Q: What is the Rf value of each amino acid observed?

A: Rf ( retardation factor):- It is defined as the ratio of distance traveled by the centre of a spot (…

Q: A test protein contains the following 4 ratios of its amino acid content (mg/g) vs the amino acid…

A: The amino acid score along with protein digestibility is a procedure to determine whether a protein…

Q: At high salt concentration, proteins are less soluble. Changing the solubility of a protein based on…

A: Salting out procedure can be used to precipitate large biomolecules, such as proteins or DNA. The…

Q: In Cystic Fibrosis, what are the normal and mutated protein function

A: Cystic fibrosis is an autosomal recessive genetic disorder which affects the lungs and also the…

Q: If 1ml of an unknown sample of protein is diluted to 10ml, and a 0.5ml portion of the diluted sample…

A: Lowry method is used for estimating the protein concentration in the unknown sample. It uses copper…

Q: Proteoglycans are often made up of glycosaminoglycans (chondroitin sulfate, keratan sulfate, and…

A: On the basis of composition, proteins can be either simple or conjugated. Conjugated proteins…

Q: Cut the following protein with the Serine Proteolytic enzyme Trypsin. How many peptide fragments are…

A: Proteins are unbranched polymers constructed from 20 standard α-amino acids. They have four levels…

Q: Given the following information, which protein would be most difficult to isolate from protein X…

A: Salting out is a method of separation of protein mixtures that is based on protein solubility. The…

Q: What are carrier proteins? Explain its types with example

A: Protein is a macronutrients which is necessary for building of muscle mass.It is commonly present in…

Q: What contributes to the asymmetric protein distributions ?

A: The lipid bilayer (or phospholipid bilayer) is a thin polar membrane made of two layers of lipid…

Q: an protein has a molecular wieght of 90 KD when purified by gel filtration and has a molecular…

A: Biomolecules are the compounds that are necessary for different biological processes occurring in a…

Q: A stock solution contains 0.2 mg/mL protein. From this stock solution, 179 uL is diluted with 1.1 mL…

A: Proteins are composed of amino acid monomers. The protein concentration can be measured in a…

Q: If 1ml of an unknown sample of protein is diluted to 10ml, and a 0.5ml portion of the diluted sample…

A: The Lowry method of protein quantification's main governing principle depends on peptide nitrogen…

Q: How many cysteines are present in the amino acid sequence you were given? A.45 B.12 C.60 D.10…

A: Cyteines are one of the 20 amino acids which are ribosomally incorporated into proteins. This amino…

Q: does it mean for a protein to be denatured

A: Protein denatured means the activity of protein has been changed or stop by changing in protein…

Q: You have a mixture of positive, negative and neutral proteins. In order to obtain a pure positively…

A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Q: how can one induce protein denaturation: select one or more

A: Protein denaturation is the process by which the protein molecule unfolds from its highly ordered…

Q: Is there a difference in the effects of different salt solutions on protein denaturation? Why?

A: Yes, there is a difference in the effects of different salt solutions on protein denaturation.…

Q: Do you think there will be a significant difference in the number of cfus/g enumerated from the…

A: A colony-forming unit is defined as the unit used in microbiology to measure the number of viable…

Q: An effort is usually made to purify a protein fırst, before its characteristics are determined. True…

A: Purification of a protein is done to get the interested protein in as native form it can be.…

Microbial Taxonomy

Taxonomy is the science of naming, classifying, and describing organisms. The taxonomy will include all the plants, microorganisms, and animals on this planet. Carl Linnaeus coined the taxonomy term. He is known as the father of taxonomy. Microbial taxonomy is a criterion of grouping microorganisms into groups based on their similarities and past relatedness. Microorganisms belonging to the same group are differentiated from the others via grouping into categories. This differentiation makes the study of microorganisms easy. There are different types of microbial taxonomies like Numerical and Molecular taxonomy. The study of microorganisms is known as microbiology. Microbiology is a broad term that takes into account microbial cultures, taxonomic studies, evolutionary relationships. Microbial interaction and diversity are huge data that needs to be compiled under the subject of microbiology. Microbiology covers various organisms like bacteria, fungi, algae, plants, animals, etc.