If you were to analyze three separate colonies from the plate above, would you expect them all to be the same species of bacteria?  Why or why not?

Q: Does each colony represent a pure colony? How will you prove your answer? Explain why an isolated…

A: A "colony" is a collection of bacteria, fungus, and other microbes cultivated on a solid agar media…

Q: You’ve collected a soil sample from an unexplored island and cultured a new bacterial species by…

A: Introduction Bacteria are single-celled organisms with a small size. Bacteria can be found…

Q: . If you wanted to determine the number of endospores in a sample of soil, what could you do before…

A: Endospores are defined as the differentiated cell formed or produced within cells of a particular…

Q: You culture an unknown bacterial species on MSA media.  The organism grows well, and the media turns…

A: MSA is a type of medium that is selective as well as differential and is extensively used in…

Q: How would you record your observation of a plate containing 305 colonies?A plate with 15 colonies?

A: A microbial colony is the population of a single type of bacteria / microorganisms, which formed…

Q: The number of bacteria in a culture every 2 hours and 30 minutes. If the initial number of Bacteria…

A: In the question, we are given: Generation time of bacteria: 2 hours and 30 min.  Initial number of…

Q: The laboratory that you are working in does not have a microscope or motility medium. Now, how will…

A: The motility of a bacterium is determined by the presence and absence of flagella. The flagellum is…

Q: You perform a quadrant streak and isolate a single colony which contains two distinct species of…

A: Quadrant streaking is a technique for microbial inoculation in which a single colony is isolated on…

Q: Suppose you have two organisms in a mixture and Organism A is 1,000 times more abundant than…

A: Spread plate technique is generally used to isolate individual colonies from a bacterial culture. It…

Q: The colonies growing on the surface of solid media represent: Select one: a. A group of different…

A: The CFU/ml are often calculated using the formula:cfu/ml = (no. of colonies x dilution factor) /…

Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…

A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…

Q: Why is oil immersion necessary to see bacteria in the laboratory?

A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…

Q: Which of the microscope objectives is most satisfactory for studying bacteria? Why?

A: For studying bacteria , under the microscope , 100X objective oil immersion lens is generally used…

Q: What do you mean by “colony” of microorganisms?

A: The earth ecosystem is full of various organisms. These organisms are prokaryotes or eukaryotes. The…

Q: You prepared another slide to visualize the bacteria under the oil immersion objective lens, but you…

A: In microscopy, oil immersion is a technique used to increase the resolving power of a microscope.…

Q: Why do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown…

A: Endospores are dormant, tough, vegetative structure produced by the bacteria from the Firmicute…

Q: Is darkfield or brightfield better at viewing bacteria? Why?

A: Bacteria are tiny microscopic groups of organisms that are not visible through naked eyes. Special…

Q: What is not part of a Bacterial cell base on the picture?

A: All the living organisms are made up of cells. These are the functional units of life that have…

Q: Can the presence and absence of capsules in bacteria be used to identify unknown bacteria? YES OR…

A: Glycocalyx is present external to the cell wall as an outermost covering of bacteria. It protects…

Q: What are the reason why scientist cannot provide the exact number of bacteria existing on earth?…

A: Bacteria have existed from very early in the history of life on Earth. Bacteria fossils discovered…

Q: You are using bacterium E.coli to synthesize a protein from recombinant DNA. The protein remains in…

A: Escherichia coli is one among the organisms of selection for producing recombinant proteins. It's…

Q: temperature and light source affect the number and type of colonies that can be found in the…

A: Microbes such as bacteria, fungi, viruses are grown at the earth's surface. The atmosphere provides…

Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…

A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…

Q: Suppose you performed a Gram stain on a sample, from a pure culture of bacteria and you observed a…

A: Gram staining is a method that use dye to stain the bacteria and the color helps them to distinguish…

Q: Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the…

A: The wet mount is the most basic method of preparation, in which the specimen is placed on the slide…

Q: If you are to invalidate the abiogenesis theory using Louis Pasteur’s experiment, how will you…

A: The oldest surviving terrestrial rocks, about 4.3 billion years old, contain no definite trace of…

Q: Is it likely that a pure culture could be isolated from the human body? Why or why not?

A: In microbiology the pure culture is a laboratory culture that contains a single species of organism.…

Q: You use a spectrophometer and estimate the concentration of bacteria in a urine sample to be 4.6 x…

A: dilution factor  =10^-6

Q: Why can you see organisms now, but couldn’t see them when you first swabbed the plate?  A bacterial…

A: Bacteria are microscopic, single-celled organisms that can be found in diverse kinds of…

Q: According to these images and your own knowledge, can you make a biological drawing of bacteria in…

A: Yoghurt is a nutrient which is rich in lactobacillus (lactic acid bacteria) . Convert milk into…

Q: What is the importance of describing the morphological characteristics of bacterial colonies when…

A: The characteristics of colony that is formed by the bacteria are - colour, opacity, shape,…

Q: How would the slide appear if the bacteria were stained with safranin, a basic red dye?

A: The appearance of bacteria, when stained with safranin, is to be determined.

Q: In the Griffith experiment, why didn't the bacteria from rough colonies harm the mice? Because they…

A: Bacteria like Streptococcus do not have membrane-bounded organelles, can reproduce individually,…

Q: If you do not see single colonies on some of the plates, explain what might have happened to the…

A: A bacterial colony is a mass of bacterial cells originating from mother cells, they grow on solid…

Q: If the original number of cells in a sample was 12,000, how many bacteria will be present in 12…

A: The proliferation of bacterium into two genetically identical daughter cells, through the process of…

Q: Would this bacteria be classified as gram negative or gram positive? Describe the shape and…

A: Gram staining is a simple technique, in which two categories of bacteria are identified based on…

Q: Why was Van Leeuwenhoek's discovery of the unknown such a milestone achievement for the progress in…

A: Antonie Van Leeuwenhoek made single lens microscope on which he did his first observations of…

Q: You use the Gram staining procedure to stain a bacterium that lacks a cell wall. Would you consider…

A: The stirring process is very much important for visualisation and identification of any living…

Q: If you obtain 35 colonies after spread plating 1 ml of a 1:500 dilution of lake water, what is the…

A: A dilution can be defined as a method of addition of more solvent to a solution of a definite…

Q: If you had a liquid culture containing three different species of bacteria, how could the use of a…

A: A microbiological culture, also known as a microbial culture, is a technique for multiplying…

Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…

A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…

Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?

A: Introduction  We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…

Q: When the microbes were exposed to different temperatures how was it possible to see minimal growth?…

A: Microorganisms are divided into different groups depending on their optimum growth temperatures.…

Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…

A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…

Q: Why does a streaking method used to inoculate plates result in isolated colonies? (Explain…

A:

Q: Why is 1857-1914 considered as the golden age of microbiology?

A: Microbiology helps us to identify the microorganisms that exist in food. With a better understanding…

Q: What would happen if the enrichment method in the isolation of bacteriophage was omitted?the…

A: The bacteriophages are bacteria that are infected by a virus are known to exist in all possible…

Q: A 2x10-6 dilution of your bacterial culture yielded 10 colonies. What was the cell density (in…

A: The purpose of dilution of the bacterial culture before plating is to ensure that the cells are…

Q: how else could bacteria in soil be characterized and identified other than gram staining

A: Gram staining method is one of the important ways to identify bacteria. This principle is based on…

Q: In a serial dilution, you plate 1 ml of a 1/1000 dilution of a bacterial culture and find 25…

A: Serial dilution of the samples is used to calculate the number of microorganisms in a sample.…

Bacterial Morphology

The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology. 

Bacterial cell structure

Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.