You use the Gram staining procedure to stain a bacterium that lacks a cell wall. Would you consider the bacterium appear Gram positive or Gram negative ? And why?
Q: How can natural pigments produced by bacteria reveal their Gram stain reaction? O Natural pigments…
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Q: Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria…
A: The gram-positive and negative cells differ in their cell wall composition.
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Q: Why are bacterial cells generally stained for microscopic viewing?
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Q: What is the importance of Gram-staining
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Q: When performing a gram stain, what does it tell us about the bacteria and the cell wall speficially?
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Q: The Gram Stain Build the gram negative structure
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Q: ll might appear Gram negative?
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Q: On the basis of the reaction in the Gram stain, bacteria can be divided into which two major groups?
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Q: The use of the Gram stain in microbiology is important because it differentiates A) Bacteria from…
A: To find the best option among the four that related to use of gram stain in microbiology.
Q: Would this bacteria be classified as gram negative or gram positive? Describe the shape and…
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Q: You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look…
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Q: Explain in your own words what you know about Gram positive and Gram negative bacteria and why they…
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Q: What can happen to cause gram-positive bacteria to appear gram-negative?
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Q: Why do we stain some organisms before viewing them under a microscope? (see top of p 14)
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- What are the structural differences between Gram-positive and Gram-negative bacteria cell walls? Can you explain what would happen to the cell wall's physical integrity while applying the Gram staining technique?Suppose you are viewing a Gram-stained field of red rods and purple cocci through the microscope. What do you conclude?if a bacteria under observation in a microscope appears to be blurry, what measures are needed to do in the microscope in order to see and identify it clearly?
- Why do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown bacteria is a Gram-negative rod. You decide to perform an endospore stain, thinking it will help you with the identification of the organism. What will you see, and why?Both stained and live organisms are viewed using a microscope, but what is the purpose/benefit of staining a microorganism versus not staining it?After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in your slide under the microscope, what could be the mistake you have done? What can you observe when you view a Gram stained-mixture of bacterial specimen?
- Why is it important to perform a gram stain for any type of bacteria? After performing the gram stain method what would be the next step? (Think in terms of the real-world situation) Explain.An acid-staining method was performed on a bacterial smear using the dyes carboifuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following couid explain why only blue cells were present?The laboratory that you are working in does not have a microscope or motility medium. Now, how will you determine if a disease-causing bacterium is plants is motile or non-motile?
- One of your classmates performed a gram stain on Pseudomonas aeruginosa and found variable gram staining, with only some of the cells gram positive. What should your classmate have found ? What errors could have contributed to the variable result?You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? a)red/pink b)purple c)colorlessIn the Gram staining procedure, how do the dyes able to distinguish Gram positive from Gram negative bacterial cells? What can you observe when you view a Gram stained-mixture of bacterial specimen?
