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- What is the clinical significance of ESR determination? Differentiate Wintrobe from Westergreen regarding accuracy of the test in a tabulated order. NOTE: Kindly asnwer all the questions. Thank you!Please explain and cite references if possible. Thank you! 1. Explain why stool specimen must not be frozen nor placed in incubators.Procedure:1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva toeach tube. Mix thoroughly.2. Place the first tube in ice water, the 2ndtube leave at room temperature, the 3rdtube in 40°C , the 4thtube at 60°Cwater bath and the 5thtube boil for 2minutes..3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4thtubeallows to stand for 30 minutes after heating for 2 minutes.4. Test the contents of each tube with iodine and benedict’s tests.
- Activity 13 Urine Culture Inoculating Urine with a calibrated loop The number of microorganisms per milliliter recovered on urine culture can aid in the differential diagnosis of urinary tract infection (UTI). Plastic or wire inoculating loop available commercially, have been calibrated to deliver a known volume of liquid when handled correctly, thus enabling the microbiologist to estimate numbers of organism in the original specimen based on colony forming unit (CFU) of growth on cultures. PROCEDURE: 1st day 1. Gently swirl the specimen bottle to mix the urine specimen. 2. Label all plated media with name of patients, clinical specimen used and date. Label at the bottom of plates and not on the cover. 3. Obtain a disposable calibrated loop. 4. Dip the loop straight into the urine specimen so that the loop part is completely covered. Withdraw straight out. See fig. 16-18 5. Inoculate blood agar plate (BAP) as shown in fig.16-19 6. Incubate at 35 – 37˚C for 18 – 24 hours. 7. Dispose…Please show me working, this is a pharcy calculation questiondescription of a the oxidase test
- Procedure 6: Respiration (Aerobic) 1. Obtain a small test tube of phenol red and carefully place six peas into the test tube with the dye. 2. Let the peas respire for 10-15 minutes. QUESTION 1. What color does the phenol red change to? Why?FOLIN-WU METHOD a. What is the type of blood sample used and how much of it is added to distilled water? b. What are the protein precipitants used and indicate the specific concentration of each? c. What is the color of the precipitate obtained? d. If the desired color of the mixture(blood sample and reagent mixture) is not achieved, what chemical is added drop by drop to obtain the desired color? Specify the conc.DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?
- Indole test what the result shows, what the result means, thank you!EXPERIMENT :DETERMINATION OF CHEMICAL OXYGEN DEMAND (COD) 1. OBJECTIVES To determine the chemical oxygen demand of the given wastewater samples. 2. PROCEDURE Preparing the Sample 1. Open a reaction vial with a white cap and add the specified volume. (Ensure that appropriate personnel safety equipment is used.) LR-/MR: 2 ml water sample HR: 0.2 ml water sample 2. Prepare a blank by using deionised water (TOC-free) instead of the sample (LR/MR: 2 ml, HR: 0.2 ml). 3. Replace the cap tightly. Invert the vial gently several times to mix the contents (The vial will become hot during mixing!) and digest the vials for 120 minutes in the reactor at a temperature of 150°C. 4. Remove the vials from the reactor and allow them to cool down to 60°C or less. Mix the contents by inverting each vial several times while still warm. Then allow the vials to cool to ambient temperature before measuring. 3:Results Sample COD reading (mg/L) Leachate 216 Factory 423…Catalase Yeast Test Questions: 1. Why is it important to graph volumes that did not include the initial volume? 2. How might the temperature of the yeast mixture affect a reaction? 3. What effect would increasing the amount of yeast have on a reaction?