In a column chromatography experiment. After purifying compounds by column chromatography, what are two other methods that you can use to confirm the identity of the compounds in each fraction? (other than TLC!) make sure to explain aswell. Thank you
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In a column chromatography experiment. After purifying compounds by column chromatography, what are two other methods that you can use to confirm the identity of the compounds in each fraction? (other than TLC!) make sure to explain aswell. Thank you
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- In this experiment, you will be performing TLC on your 'crude' and 'recrystallized' biphenyl samples. How many spots would you expect to see in each lane? Explain your answer. 2. If you perform pipette (column) chromatography on the mixture containing biphenyl and organic dye, which compound would you expect to elute from the column last. Explain your answer.The compounds biphenyl and naphthalene are presented in a mixture. Can hexane be used as a solvent to separate the compounds by column chromatography with silica gel as the stationary phase? Why or why not.Are column chromatography, TLC and melting point each used to purify or characterize (identify) a compound?
- Using reversed phase chromatography in order to separate chloroquine and hydroxychloroquine. Which will be the first one to elute out of the column? Or both will elute the same time?3. If ferrocene was contaminated with some acetylferrocene after the column, what couldyou do to purify it further?4. Could you use a column to separate a mixture of 3 different compounds? Why or whynot?5. Reverse phase chromatography is a type of column where the stationary phase is verynonpolar. If you were to run a reverse phase column on ferrocene and acetylferrocene,in what order would they elute? How could you test the purity of your samples after thecolumn?1) When you try separating an unknown mixture by normal-phase liquid chromatography with 50% hexane and 50% methyl t-butyl ether, the peaks are too close together. (not resolved) Should you use a higher or lower concentration of hexane in the next run?
- In kool aid chromatography, why does reducing the dilution of 2-propanol result in its decrease in polarity?Indicate whether TLC (thin-layer chromatography) or column chromatography is more appropriate for the following:a. This is an analytical method for separating compounds of amixture.b. The solvent front moves downward.c. This is a preparative method for separating a mixture ofcompounds.A thin-layer chromatography (TLC) experiement was run using three compunds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with the three compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?
- Of Aspirin, ethanol, acetaminophen, ethyl acetate, hexane, and water which could go undetected during HPLC analysis, but could result in a lower than expected melting point, why are they not detected by HPLC analysis?Help please : When comparing HPLC with Gas Chromatography , Gas Chromatography analysis may require a chemical derivatisation step before analysis of your sample. Define chemical derivatisation, state why it is needed and briefly describe the methods that can be used to make your sample more appropriate for GC ?To separate a mixture of compounds containing the following two compounds column chromatography was used by a student. Stationary phase was silica gel and mobile phase was 95 % ethyl acetate and 5 % Hexane mixture. Which compound elutes first through the column? Why