Q: What are the three basic stages of transcription? Describe what happens at each stage.
A: Transcription is process of copying genetic information from one strand of DNA into RNA. It occurs…
Q: The recognition sequence to which RNA polymerase binds at the initiation of transcription is found…
A: Ans- False The recognition sequence to which RNA polymerase binds at the initiation of transcription…
Q: The following diagram represents one of the Christmas-tree-like structures shown in Figure On the…
A: DNA molecule is composed of two polynucleotide chains and coil in a double helix. The molecule…
Q: Match each of the following to cis-acting or trans-acting. If you are in doubt, try drawing out a…
A: Operon is a set of structural genes in a bacterial cell, along with common promoter and other…
Q: Explain why the glucocorticoid receptor binds next to the core promoter of some genes, but not next…
A: Gene expression is a process in which the information present in the particular sequences is…
Q: What is the difference between an operator and a promoter?
A: Replication is the process by which DNA makes identical copies. Transcription is the formation of…
Q: What is meant by Rho dependent and independent transcription termination. What is the differences?…
A: Termination in transcription is a point when enzyme stops adding nucleotides to the growing RNA…
Q: What is the function of the promoter
A: Promoter is the site on DNA where proteins such as RNA polymerase and some transcrption factors…
Q: What would you expect to happen if you delete an important portion of a promoter?
A: Promoters are immediately upstream from the gene and play a crucial role in determining whether…
Q: In general, why is it important to regulate genes? Discuss examples of situations in which it would…
A: Genes are sets of nucleotides that codes for a particular protein. The genes have to be expressed…
Q: If a mutation occurred in the pRM promoter that rendered this promoter as ‘nonfunctional and unable…
A: Promoter sequence is present in the upstream of the gene sequence , is the part of dna where rna…
Q: If a gene is repressible and under positive control, what kind of effector molecule and regulatory…
A: Gene regulation vary in prokaryotes and eukaryotes. Prokaryotic genes regulation may administrated…
Q: What are two subcategories within eukaryotic promoters ?
A: Promoters are regions on the DNA where different proteins bind to initiate transcription, the…
Q: Give two reasons why both the strands are not copied during transcription?
A: Processes of synthesizing RNA from DNA with the help of an enzyme RNA polymerase is termed as…
Q: Many genes generate different products depending on the type of cell expressing the gene. How is…
A: The process of generation of different protein products from the same pre-mRNA transcript is called…
Q: Why are miRNAs called noncoding RNAs? Explain how they participate in gene regulation.
A: Answer- miRNA are non coding RNA because it does not code for any amino acid to form proteins.
Q: a. Use circles for activators and squares for repressors. Draw the shapes bound to each promoter…
A: Transcription - The process by which the information on one strand of DNA is copied to mRNA is…
Q: Discuss the structure and function of regulatory elements. Where are they located relative to the…
A: A eukaryotic organism is made up of several genes. It is essential to ensure that not all genes are…
Q: Explain what are dispersed core promoters
A: Transcription is the process by which the information contained in DNA is transcribed to a…
Q: Contrast the two types of transcription terminators in E.coli.
A:
Q: Define promoter region and list three of its properties.
A: Introduction The region with the specific DNA sequence, right upstream to the transcription starting…
Q: Describe the characteristics of a eukaryotic promoter for a protein-encoding gene.
A: Transcription is the cycle by which the data in a strand of Deoxyribosenucleic acid (DNA) is…
Q: How many types of promoters are there?
A: Promoters are DNA sequences that determine where the gene transcription by RNA polymerase begins.…
Q: Why is it important for a transcription factor/activator to have a high affinity for a specific DNA…
A: Transcription is a metabolic process in which DNA sequence of the gene is copied into an RNA…
Q: An enhancer, located upstream from a gene, has the following sequence: 5′–GTAG–3′ 3′–CATC–5′ This…
A: An enhancer is a short segment of Deoxy ribonucleic acid (DNA), which binds to activator proteins so…
Q: A mutation within the promoter region can alter transcription of a gene. Describe how this can…
A: A promoter is a transcriptional unit which is located towards 5' end (upstream) of the structural…
Q: What is dominant control mechanism of gene expression? explain the following image.
A: Dominant alludes to a connection between two adaptations of a gene. In case one is dominant, the…
Q: Explain (in one or two lines) the function of the followings:(a) Promoter(b) tRNA(c) Exons
A: The process of DNA based gene expression in which a particular sequence of DNA molecules are copied…
Q: What is the difference between a promoter and an enhancer?
A: Promoter and enhancer are the regulatory elements of the genes. They don't produce any RNA or…
Q: Explain how an α helix in a transcription factor protein is able to function as a recognition helix.
A: Transcription is the first step in central dogma of protein synthesis. It involves formation of…
Q: An activator has bound to an enhancer region close to the promoter for gene A. What will this mean…
A: For the synthesis of a protein, the process of transcription and translation takes place.…
Q: The promoter for the above transcription unit is located on the A Left B. Right C Arrow 1 position…
A: A promoter is a region of DNA where RNA polymerase ties to start transcription. A terminator is a…
Q: You are studying a bacterial plasmid that contains 3 operons and 9 genes. How many transcriptional…
A: In bacteria, similar and related genes are often found in a cluster on the chromosome, where they…
Q: Give two reasons why both the strands of DNA are not copied during transcription.
A: Gene expression is a process by which the genes are turned on to form RNA and proteins.…
Q: Explain how a gene knockdown reagent, such as RNAi or a morpholino, could interfere with expression…
A: Gene knockdown is a mechanism or technique that helps in the reduction of gene expression. It can…
Q: Explain what are dispersed core promoters ?
A: Transcription is the process of transferring information from DNA to a single-stranded molecule…
Q: "Promoter is unmethylated, and gene can be transcribed" Illustrate this diagramtically ?
A: A gene is a DNA-based functional heredity unit that delivers instructions for the production of RNA…
Q: For the ovalbumin gene shown, indicate the locations of the following: (a) transcription start site,…
A: The transcription start site is the site on the DNA from which the first RNA nucleotide (+1) is…
Q: Can methylation state of the promoter influence the level of gene expression? Briefly describe how…
A: In eukaryotes, DNA methylation occurs by an enzyme known as DNA methyltransferase. Where the…
Q: Is it possible to induce the protein expression of a yeast gene from a prokaryotic expression vector…
A: Yes this is possible... First we isolate the yeast species which are needed for protein expression.…
Q: The specific sequence component of the bacterial promoter located 10 base pairs upstream of the a…
A: A promoter is a region of DNA where transcription of a gene is initiated. A promoter is a regulatory…
Q: Describe the characteristics of a bacterial promoter.
A: An operon is a functional unit of DNA (Deoxyribonucleic acid) containing a cluster of genes under…
Q: In eukaryotes, transcription factors can directly affect the ability of TFIID (TATA binding protein)…
A: A transcription factor is also known by the name of sequence-specific DNA binding factor. It is…
Q: you design an ideal promoter for a gene to be highly expressed in E. coli cells? Describe strategies…
A: Which Organism to Use? The preference of the host molecular whose protein synthesis equipment will…
Q: What occurs when the appropriate signals turn on the promoter?
A: A promoter is a DNA sequence towards which proteins attach to start the process of transcription of…
Q: In the laboratory, you want to study protein that is normally toxic to E. coli cells. You wish to…
A: AraBAD promotor is a structural gene of L-arabinose operon in E.coli. AraBAD basically are 3…
Q: Which feature of promoters can be found in both prokaryotes and eukaryotes? a. GC box b. TATA box c.…
A: Transcription is a process by which the information a strand of DNA is copied into a new molecule of…
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- In general, why is it important to regulate genes? Discuss examples of situations in which it would be advantageous for a bacterial cell to regulate genes.On Figure , indicate the locations of the promoters and terminators for genes a, b, and c.Compare and contrast two mechanisms for transcriptional termination in bacteria.
- Explain why large amounts of transcript result when a bacterium with a lac Operon is in a medium with no glucose and an abundance of lactose.a. Use circles for activators and squares for repressors. Draw the shapes bound to each promoter region. b. Under the word gene write “ON” or “OFF” to indicate if the gene is transcribed or not under the different conditions.The following diagram represents one of the Christmas-tree-like structures shown in Figure On the diagram, identify parts a through i. Q. Approximate location of the promoter
- A crucial step in the regulation of many bacterial genes is the binding of RNA polymerase to DNA at the promoter. Why might it be advantageous for bacteria to regulate the expression of their genes at this particular step?you design an ideal promoter for a gene to be highly expressed in E. coli cells? Describe strategies and explainWhat is the regulatory region of the Trp operon that is involved in alternative transcription termination mechanism? Choose from the options below. Attenuator Promoter Leader sequence Repressor Operator