In the laboratory, you want to study protein that is normally toxic to E. coli cells. You wish to grow this protein in E. coli and purify it from E. coli. Your advisor suggests cloning the gene into an expression vector that uses the araBAD promoter. Explain why is it ideal to use the araBAD promoter for expression of your gene of interest in E. coli cells?

In the laboratory, you want to study protein that is normally toxic to E. coli cells. You wish to grow this protein in E. coli and purify it from E. coli. Your advisor suggests cloning the gene into an expression vector that uses the araBAD promoter. Explain why is it ideal to use the araBAD promoter for expression of your gene of interest in E. coli cells?

Name: In the laboratory, you want to study protein that is normally toxic to
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Description: In the laboratory, you want to study protein that is normally toxic to E. coli cells. You wish to grow this protein in E. coli and purify it from E. coli. Your advisor suggests cloning the gene into an expression vector that uses the araBAD promot

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 8TYU: Through alternative splicing, eukaryotes (a) reinforce gene inactivation (b) prevent transcription...

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(a) Did deletion of any of the possible control elements cause areduction in reporter gene expression? If so, which one(s), and howcan you tell? (b) If loss of a control element causes a reduction ingene expression, what must be the normal role of that controlelement? Provide a biological explanation for how the loss of sucha control element could lead to a reduction in gene expression.
In the bacteriophage T7 system used to express recombinant proteins, the gene of interest is fused to T7 promoter and T7 RNA polymerase is separately cloned into the same cell. What is the main reason this system uses T7 RNA polymerase instead of relying on the bacterial RNA polymerase? To restrict the expression of bacterial protein expression To enhance the amount of recombinant protein expression To enhance the expression of bacterial protein expression To restrict the amount of recombinant protein expression To enable the expression of T7 viral protein expression
you design an ideal promoter for a gene to be highly expressed in E. coli cells? Describe strategies and explain
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The insertion of transposable elements into genes can alter the normal pattern of expression. In the following situations, describe the possible consequences on gene expression.a. A LINE inserts into an enhancer of a human gene. b. A transposable element contains a binding site for a transcriptional repressor and inserts adjacent to a promoter. c. An Alu element inserts into the 3′ splice (AG) site of an intron in a human gene. d. A Ds element that was inserted into the exon of a maize gene excises imperfectly and leaves three base pairs behind in the exon. e. Another excision by that same Ds element leaves two base pairs behind in the exon. f. A Ds element that was inserted into the middle of an intron excises imperfectly and leaves five base pairs behind in the intron.
. Another class of suppressor mutations, not describedin the chapter, are mutations that suppress missensemutations.a. Why would bacterial strains carrying such missense suppressor mutations generally grow moreslowly than strains carrying nonsense suppressormutations?b. What other kinds of mutations can you imagine ingenes encoding components needed for gene expression that would suppress a missense mutationin a protein-coding gene?
What role does an operator sequence serve in bacterial gene expression regulation? Describe one change in bacterial cells that can cause a repressor protein to go from inactive to active or from active to inactive.
Some vectors used in cloning experiments contain bacterial promotersthat are adjacent to unique cloning sites. This makes it possible to insert a gene sequence next to the bacterial promoter and express the gene in bacterial cells. These vectors are called expression vectors. If you wanted to express a eukaryotic protein in bacterial cells, would you clone genomic DNA or cDNA into the expression vector? Explain your choice.
An enhancer, located upstream from a gene, has the following sequence: 5′–GTAG–3′ 3′–CATC–5′ This enhancer is orientation-independent. Which of the following sequences also works as an enhancer? A. 5′–CTAC–3′ 3′–GATG–5′ B. 5′–GATG–3′ 3′–CTAC–5′ C. 5′–CATC–3′ 3′–GTAG–5′ C15.
Can methylation state of the promoter influence the level of gene expression? Briefly describe how gene expression changes based on the promoter methylation level
You have isolated different mutants (reg1 and reg2) causing constitutive expression of the emu operon (which has genes emu1 and emu2). One mutant contains a defect in a DNA-binding site, and the other has a loss-of-function defect in the gene encoding a protein that binds to the site. Is the DNA-binding protein a positive or negative regulator of gene expression?
You are teaching a class on the regulation of eukaryotic gene expression. In order to demonstrate this complex process, you decide to draw for the class a typical eukaryotic gene/transcription unit with its major regions, such as the promoter regions, where the RNA polymerase II and transcription factors would bind From the list given - choose all components that you think are part of a typical eukaryotic gene From the list given - choose all the regulatory sequences that you think would control the expression of this eukaryotic gene From the list given - choose all of the regulatory proteins that would bind the eukaryotic gene to control its expression