In column chromatography, the following statements are true except-* The stationary phase is held in a narrow tube through which the mobile phase is forced under pressure The effectiveness of the chromatographic column in separating two solutes depends in part on the relative rates at which the two species are eluted The stationary phase is supported in a flat plate or in interstices where the mobile phase moves through by capillary action or under the influence of gravity Predict the order of elution of the following components if a normal phase column and hexane:methylene chloride mobile phase was used in the separation. (A.) Propanone (B.) Propanoic acid (C.) Methyl ethanoate BCA ABC ACB The following are true for the observed tailings and fronting in a chromatogram, except- Fronting and tailing leads to a good separation and reproducible elution times Tailing is observed when the tail appears to the right of the chromatogram and front is steepened while fronting is observed when the tail is steepened Common cause of fronting and tailing is a distribution constant that varies with concentration

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter33: Automated Methods Of Analysis
Section: Chapter Questions
Problem 33.8QAP
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In column chromatography, the following statements are true except-*
The stationary phase is held in a narrow tube through which the mobile phase is
forced under pressure
The effectiveness of the chromatographic column in separating two solutes depends
in part on the relative rates at which the two species are eluted
The stationary phase is supported in a flat plate or in interstices where the mobile
phase moves through by capillary action or under the influence of gravity
Predict the order of elution of the following components if a normal
phase column and hexane:methylene chloride mobile phase was used in
the separation. (A.) Propanone (B.) Propanoic acid (C.) Methyl
ethanoate
BCA
ABC
ACB
The following are true for the observed tailings and fronting in a
chromatogram, except-
Fronting and tailing leads to a good separation and reproducible elution times
Tailing is observed when the tail appears to the right of the chromatogram and front is
steepened while fronting is observed when the tail is steepened
Common cause of fronting and tailing is a distribution constant that varies with
concentration
Transcribed Image Text:In column chromatography, the following statements are true except-* The stationary phase is held in a narrow tube through which the mobile phase is forced under pressure The effectiveness of the chromatographic column in separating two solutes depends in part on the relative rates at which the two species are eluted The stationary phase is supported in a flat plate or in interstices where the mobile phase moves through by capillary action or under the influence of gravity Predict the order of elution of the following components if a normal phase column and hexane:methylene chloride mobile phase was used in the separation. (A.) Propanone (B.) Propanoic acid (C.) Methyl ethanoate BCA ABC ACB The following are true for the observed tailings and fronting in a chromatogram, except- Fronting and tailing leads to a good separation and reproducible elution times Tailing is observed when the tail appears to the right of the chromatogram and front is steepened while fronting is observed when the tail is steepened Common cause of fronting and tailing is a distribution constant that varies with concentration
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