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- A Biuret test is a chemical test used to determine the presence of a peptide bond in a substance. Then how can Biuret test for proteins may also be extended to quantitatively measure the concentration of total protein?Denaturation of protein is a loss of protein structure. Given enough time, can all denatured protein spontaneously renature? Justify answerThe elution of a protein with an isoelectric point of 7.5, is mostly likely to be affected by a change in pH from 7.4 to 6.9 in which type of protein separation technique? a.) size exclusion chromatography b.) SDS-acrylamide gel electrophoresis c.) affinity chromatography d.) ion exchange chromatography
- match the letters of the bands to the proper protein based on the given dataWhy is a protein's structure important? What can happen if there are any changes? Provide an example and cite sources.Which of the following statements about electron microscopy are true? a) Most existing protein structures have been resolved using electron microscopy b) Electron microscopy is not used in structural biology as it can not give as high a resolution as X-ray crystallography and NMR c) The smaller a protein, the easier it is to solve its structure with electron microscopy d) By taking pictures of the same protein frozen in ice thousands of times and then adding them together, you get a high-resolution image of the protein e) The first protein structure with true atomic resolution was solved using electron microscopy last year
- can you write a paragraph on the following about sds-page & protein structure investigationWhich method for determining protein structure involves cleaving proteins into smaller polypeptides using enzymes and using performic acid and dithiothreitol (DTT) to remove disulphide bridges? a)Matrix-assisted laser desorption/ionization mass spectrometry b)Electrospray ionization mass spectrometry c)Edman's degradation d)Tandem mass spectrometry e)Enzyme protease fragmentation.Which of the following statements about electron microscopy are true?Group of answer options a.By taking pictures of the same protein frozen in ice thousands of times and then adding them together, you get a high-resolution image of the protein b.Electron microscopy is not used in structural biology as it can not give as high a resolution as X-ray crystallography and NMR c.The smaller a protein, the easier it is to solve its structure with electron microscopy dMost existing protein structures have been resolved using electron microscopy e.The first protein structure with true atomic resolution was solved using electron microscopy last year
- Suppose you have a cell-lysis sample containing genomic DNA, a complex mixture of proteins, and debris. Describe different commonly used analytical methods to i) analyze the molecular masses of the protein mixture. ii) detect the composition (what specific amino acids are there in what proportion) of a specific protein from the mixture. iii) deduce the sequence of some particular proteins from the mixture contentWhich of the following is not one of the basic features used in protein separation techniques? a. Specific binding affinity of protein b. Protein charge c. Viscosity of the protein d. Solubility of proteinYou're on duty at a prominent Metro laboratory. You mixed up a sample for protein analysis of the samples needed for forensic examination that contains pure DNA while waiting for your closest friend. You decided to use the knowledge you gained in Biochemistry to run the combined samples through a spectrophotometer to eliminate contaminated protein. Determine which of the samples should be extracted and separated for protein analysis based on the following data. Show all of your solutions. A230 A260 A280 Buffer 0.051 0.091 0.065 Sample A 1.27 4.54 2.01 Sample B 1.32 3.92 2.11 Sample C 1.95 3.88 2.04 Sample D 1.44 4.21 2.32