In the antibiotic disc diffusion study, the "Minimal Inhibitory Concentration" or MIC of the antibiotic was in the agar in the agar furthest away from the antibiotic disc O halfway between the disk and the border of the zone of inhibition directly under the antibiotic disc O at the border/edge of the zone of inhibition
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Antibiotic disc diffusion study is also known as the Kirby-Bauer method in which the susceptibility and sensitivity of an antibiotic are tested. The disc of respective antibiotics is placed on the plate where bacteria have been swabbed and grown already which after incubation may or not produce the zone of inhibition that depends upon the type of bacteria used, respective antibiotic, and dosage.
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- diameter AM=47MM TE= 27 MM both left hand side is ampicillin and right hand side is tetracycline With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. S.aureus Antimicrobial agent Interpretive categories and zone diameter breakpoints, nearest whole mm sensitive Intermediate resistant Tetracycline >19 15-18 <14Chemical modification of an antibiotic by bacteria would lead to a loss of function of the antibiotic. O True O FalseThe size of the inhibition zone depends on conditions such as: Multiple Choice The concentration of the antibiotic loaded onto the disk The solubility of the antibiotic availability of moisture The concentration of the antibiotic loaded onto the disk AND the solubility of the antibiotic
- The clear zone around an antibiotic disk Multiple Choice is a plaque indicating the bacteria got killed is a zone of inhibition is a zone of inhibition indicating resistance of microbes is a zone of inhibition indicating susceptibility of microbesSome bacteria produce the enzyme penicillinase, which breaks the beta-lactam ring in the penicillin molecule and converts it into penicilloic acid, which is harmless to bacteria. This is an example of which of the following mechanisms of antibiotic resistance? which option below is the answer? Destruction or inactivation of the antibiotic by the microbe Preventing penetration to the target site within the microbe Alteration of the drug’s target site(s) within the microbe None of the other four answers are correct Rapid ejection (pumping) of the drug out of the microbial cellWhat does the zone of inhibition imply? Does the measurement of the zone ofinhibition imply that one antibiotic is better than the other? Support your answer.
- nt ● ● S ● Paragraph F 2. To determine if the type of agar affects bacterial growth, a scientist cultures on fou different types of agar. Five petri dishes are set up to collect results: One with nutrient agar and E. coli One with mannitol-salt agar and E. coli One with MacConkey agar and E. coli One with LB agar and E. coli Styles One with nutrient agar but NO E. coli Edboth left hand side is tetracycline and right hand side is ampicillin With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. Am=17mm Te=23mm E.coli Antimicrobial agent sensitive Intermediate resistant Interpretive categories and zone diameter breakpoints, nearest whole mm Ampicillin >17 14-16 <13 Tetracycline >15 12-14 <11The Kirby-Bauer test is useful for determining Multiple Choice the minimum inhibitory concentration of an antibiotic. whether side effects will result from use of an antibiotic. the sensitivity of a microorganism to an antibiotic the right dose of an antibiotic for treatment of infection
- A researcher is planning to plant antibiotic-resistant Escherichia coli pure bacteria sample in antibiotic (ampicillin) solid media in his experiment. Describe each of the sterilization methods that this researcher should use while preparing this solid medium with antibiotics and in cultivation of the bacteria on the solid medium, indicating the stages at which it should be used and giving the mechanism of action.What results on a microdilution antimicrobial susceptibility test would indicate an antibiotic would be an effective treatment for an infection? O a. Growth next to the antibiotic O b. High optical density in the well with inoculum and antibiotic O c. Low optical density in the well with inoculum and antibiotic O d. No growth next to the antibiotic disk Clear my choice Next pageuse these OD numbers to plot growth curve for E. coli K12- and estimate generation time for this culture. Table 1. Absorbance (O.D.600) measured for growing culture of E. coli K12 (time course) Incubation time O.D.600 Incubation time O.D.600 O min 3 hrs 3 hrs 30 min 4 hrs 4 hrs 30 min 5 hrs 5 hrs 30 min 0.11 1.75 30 min 0.20 1.94 1 hr 1 hr 30 min 2 hrs 2 hrs 30 min 0.27 2.24 0.51 2.48 2.31 2.19 0.65 1.30