In the context of chromatographic separation, discuss Eddy diffusion, longitudinal diffusion, and mass transfer, and the effect that each makes in determining the height equivalent to a theoretical plate. Also show how to obtain the maximum efficiency.

Q: Account for the versatility of thin-layer liquid chromatography.

A: Answer - Thin layer liquid chromatography - Thin-layer  liqid chromatography is a chromatography…

Q: What is the major driving force in chromatographic separation? difference in volatility in liquid…

A:

Q: Identify The most commonly used stationary phase in paper chromatography.

A: The question is based on the concept of chromatography. It is a technique of separation and…

Q: Which of the following factors DOES NOT affect retention time of a sample undergoing normal phase…

A:

Q: Why is longitudinal diffusion a more serious problem in gas chromatography than in liquid…

A: The given statement is "the longitudinal diffusion is a serious problem in gas chromatography than…

Q: How are the column efficiency in a GC separation affected by the carrier gas flow?

A: How are the column efficiency in a GC separation affected by the carrier gas flow? explanation is…

Q: Explain  what is the van Deemter equation: Assumptions, limits, and adjustment to modern high…

A: van Deemter equation is the equation which shows the relationship between plate height and mobile…

Q: How the 2D Thin layer Chromatography separation processes are worked? Please shortly wtite at your…

A: A 2D - TLC plate is a sheet of glass, metal, or plastic which is coated with a thin layer of a solid…

Q: Explain shortly how the chromatography will be influenced in a C18 reverse phase liquid…

A: “Since you have posted a question with multiple sub-parts, we will solve first three sub-parts for…

Q: You are using column chromatography to separate the four compounds drawn below. Predict the elution…

A: Carboxylic acid, ester and alcohols are oxygen containing organic compounds. oxygen is more…

Q: How the 2D Thin layer Chromatography separation processes are worked? Please shortly answer at your…

A: Hh

Q: What are  the limitations of using  molecular/size exclusion chromatography?

A: In SEC, mass is not measured so much as the hydrodynamic volume of the polymer molecules, that is,…

Q: Micellar electrokinetic capillary chromatography was conducted at pH 10 with anionic micelles and…

A: (i) In micellar electrokinetic capillary chromatography, surfactants are added to the buffer…

Q: Why do H2 and He allow more rapid linear flow rates in gas chromatography than does N2, without loss…

A: Chromatography is a analytical process of of separation of a mixture ,in which the solution is…

Q: What is the role of stationary phase and mobile phase in Chromatography? Please shortly write at…

A: Concept is based on Chromatography.

Q: What are the mandatory components required for column chromatography?

A: What are the mandatory components required for column chromatography?

Q: The following data were obtained during gas-liquid chromatography of a mixture of two volatile…

A: Given data for components X and Y:

Q: (3) A 0.35 mm diameter open tubular gas chromatography column is coated with stationary phase that…

A: Golay's equation Golay's equation is used to find the separation efficiency of open tubular column.…

Q: Describe 4 similarities and 4 differences between Thin layer chromatography (TLC) and column…

A: The chromatography technique is used to isolate the components of mixtures that have two phases, one…

Q: What are the different parts of Flash  Column Chromatography?  Please answer at your own words

A: Flash column chromatography is a method in which chemical mixtures are separated and purified by…

Q: Species A and B were separated using column chromatography. The elution time for species A (1) was…

A: The theoretical plate height H = L/N where L is the length of the column and N = (tr)2/(w/4)2 where…

Q: Column Chromatography) What complications would a dried-out column (solvent level is below the top…

A: column chromatography is the technique used to separate compounds of different polarity .In this…

Q: Suggest a reason why the optimum linear flow rate is much higher in gas chromatography than in…

A: Given:-             The optimum linear flow rate is much higher in gas chromatography than in liquid…

Q: tabulated

A: To get: Common characteristics, advantages, and disadvantages of below:- 1) Paper chromatography…

Q: Define Flash Column Chromatography? Please answer at your own words

A: The phenomenon of flash column chromatography is related to the chemical analysis method. The word…

Q: What are the properties that determine retention time in the Chromatography process?

A: In chromatography, retention time is the interval between the injection of a sample and the…

Q: 1. Define retention factor. Explain why it is or isn't an intrinsic property of a substance.  2. A…

A: TLC (Thin layer chromatography) is a technique used to separate the components in a mixture using a…

Q: A molecular exclusion column has a diameter of 7.8 mm and a length of 30 cm. The solid portion of…

A: (a)

Q: What is the purpose of derivatization in chromatography? Give an example.

A: The technique or process used for the separation of mixture of components present in the mixture is…

Q: Explain the difference between wall-coated, support-coated, and porous-layer open tubular columns…

A: In wall coated columns the stationary liquid phase rests on capillary column which in turn consists…

Q: What is the difference between a reconstructed total ion chromatogram and a selected ion…

A: Mass chromatography  is a technique used to analyze the structural features for given family of…

Q: If satisfactory quantitative data are to be obtained from chromatograms, what parameters must be…

A: If satisfactory quantitative data are to be obtained from chromatograms, what parameters must be…

Q: (3) A 0.35 mm diameter open tubular gas chromatography column is coated with stationary phase that…

A: Gas chromatography (GC) is a technique that is used for the quantitative analysis of organic…

Q: What are the disadvantages of uses the Thick layer stationary phase in Thin layer Chromatography…

A: There was so many disadvantages of uses of  thick layer stationary phase in thin layer…

Q: What is the difference between liquid-liquid and liquid-solid chromatography?

A: Chromatography is a technique that allows the identification, separation, and determination of…

Q: Explain what the following factors consist of and their influence on the chromatographic…

A: Chromatography is a technique of separation and isolation of sample compounds. It works on the…

Q: Explain how column chromatography and planar chromatography methods are performed.

A: Column chromatography: This is a type of chromatography which occurs in a glass column known as…

Q: Determine the order of inhibition time of hexane, hexanol and benzene compounds in the reverse phase…

A: Given compounds: Hexane, hexanol and Benzene. To determine: The order of Inhibition time in Reverse…

Q: The following data are for a liquid chromatographic column: Length of Packing 24.7 cm Flow rate…

A:

Q: What is the purpose of solid-phase extraction? Why is it advantageous to use large particles (50 mm)…

A: An extraction method where compounds are suspended (or) dissolved in mixture of liquid are isolated…

Q: In chromatography, why is there a need for the stationary phase to be equilibrated?

A: Chromatography is one of the laboratory technique which can be employed for the separation of the…

Q: What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly…

A: TLC

Q: enumerate the advantage of TLC methods of chromatography over column and paper chromatography.

A: Chromatography is a useful technique to precisely separate, analyse, and purify a wide range of…

Q: Gel permeation chromatography depends on the degree of species segregation of a sample

A: Given         Gel permeation chromatography depends on the degree of species segregation of a sample…

Q: With the aid of a well labelled block diagram, briefly describe the functions of the major…

A: When two or more compounds are mixed together in a specific ratio/random way, it results in the…

Q: What type of materials can be used as the stationary phase for column chromatography? O Inorganic…

A:

Q: a 150 g. dried leaves sample of Ficus religiosa was subjected to ethanol extraction for 48 h, an…

A: The technique which is commonly used in laboratory for the separation of components of mixture is…