In their studies of Streptococcus pneumoniae, members of Avery's lab demonstrated that bacteria can pick up and use DNA from the environment. This was the first documentation of the process now known as transformation. We now know that transformation a) can transfer up to 20 genes from the environment into a bacterial cell. b) can be made permanent if the stretch of DNA includes the operator sequence from an operon. Oc) occurs more readily if the bacterial cells have first been infected with a temperate bacteriophage. d) requires the presence of a geno for ampicillin resistance in the stretch of DNA. all of the above are true of transformation

In their studies of Streptococcus pneumoniae, members of Avery's lab demonstrated that bacteria can pick up and use DNA from the environment. This was the first documentation of the process now known as transformation. We now know that transformation a) can transfer up to 20 genes from the environment into a bacterial cell. b) can be made permanent if the stretch of DNA includes the operator sequence from an operon. Oc) occurs more readily if the bacterial cells have first been infected with a temperate bacteriophage. d) requires the presence of a geno for ampicillin resistance in the stretch of DNA. all of the above are true of transformation

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 11TYU

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In Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.
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In the absence of tryptophan and the promoter sequence was mutated such that the RNA polymerase can no longer bind, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the presence of tryptophan and the promoter sequence was mutated such that the RNA polymerase can no longer bind, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"]
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. What is the role of catabolite activator protein (CAP) in the lactose operon of E. coli? it prevents RNA polymerase from binding to the promoter when lactose levels are high it assists RNA polymerase in binding to the promoter when glucose levels are high it prevents RNA polymerase from binding to the promoter when glucose levels are low it assists RNA polymerase in binding to the promoter when glucose levels are low it prevents RNA polymerase from binding to the promoter when lactose levels are low
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In the presence of lactose and the promoter sequence was mutated such that the RNA polymerase can no longer bind, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the absence of lactose and the lacI repressor gene is mutated such that the repressor protein product can no longer bind to the operator, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the presence of lactose and the lacI repressor gene is mutated such that the repressor protein product can no longer bind to the operator, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"]
When lactose is first introduced into the medium, the lac operon is not induced and thus permease isn't induced. How is it possible for lactose to enter the cell before the lac operon is induced? While the lac operon is not induced yet, lactose has many different ways to get into the cells without the need of permease. While the lac operon is not induced yet, lactose breaks down outside the cell into smaller components in order to get into the cell and then the components reassemble into lactose again inside the cell so that the induction can occur. While the lac operon is not induced yet, there is always a very high level of permease and beta galactosidase in the cell. While the lac operon is not induced yet, there is full level expression most of the time which allows a sufficient amount of permease to be expressed. This allows lactose to enter the cell. While the lac operon is not induced yet, there is low level expression most of the time which…
What is a bacterial operon and the two types of it?
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