In this experiment you will:1) Perform an experiment to determine the effect that various alcohols have on the integrity ofbeetroot membranes2) Observe the structure of intact beetroot cells by preparing a slide of these cells and observingthem microscopicallyPart 1:The Effect of Different Alcohols on Membrane PermeabilityAlcohol is an organic solvent that, as you observed, disrupts biological membranes. The question youare exploring in this lab is whether there is a difference in the effect on membranes of different kinds ofaiconol. You will be testing the effect of 3 different alcohols (methanol, ethanol and n-Propanol) on thepermeability of biological membranes. The structural formulas of these three alcohols are shown below:HH.HHTH C C C-OHH C C-OHH C-OHH HHr-PropanolEthanolMethanolM.W. o .0950246.06844M.W. LM.W. 32.0186Prior to performing the experiment, identify some of the characteristics that determine permeability:What is the molecular weight (M.W.) of each of these alcohols? Fill in above.Rank these three alcohols from most polar to least polar. What criteria did you use to determinepolarity?Methanol- MOst Polar,Prapand because it has3-H 9raups and these arehddrooholoic. The longerth-ealkul Chain beomes the lesspolarEthanolpropanolteast poarDevelop a testable hypothesis about the effect of different alcohols on the permeability ofbiological membranes based on their molecular weight and / or polarity.3Revised 8/10/2016I-C-II-C I

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Asked Jul 16, 2019
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develop a testable hypothesis about the effect of different alcohols on the permeability of biological membrrane based on their weight and / or polarity

In this experiment you will:
1) Perform an experiment to determine the effect that various alcohols have on the integrity of
beetroot membranes
2) Observe the structure of intact beetroot cells by preparing a slide of these cells and observing
them microscopically
Part 1:
The Effect of Different Alcohols on Membrane Permeability
Alcohol is an organic solvent that, as you observed, disrupts biological membranes. The question you
are exploring in this lab is whether there is a difference in the effect on membranes of different kinds of
aiconol. You will be testing the effect of 3 different alcohols (methanol, ethanol and n-Propanol) on the
permeability of biological membranes. The structural formulas of these three alcohols are shown below:
H
H.
HH
T
H C C C-OH
H C C-OH
H C-OH
H H
H
r-Propanol
Ethanol
Methanol
M.W. o .09502
46.06844
M.W. L
M.W. 32.0186
Prior to performing the experiment, identify some of the characteristics that determine permeability:
What is the molecular weight (M.W.) of each of these alcohols? Fill in above.
Rank these three alcohols from most polar to least polar. What criteria did you use to determine
polarity?
Methanol- MOst Polar,Prapand because it has
3-H 9raups and these are
hddrooholoic. The longerth-e
alkul Chain beomes the lesspolar
Ethanol
propanol
teast poar
Develop a testable hypothesis about the effect of different alcohols on the permeability of
biological membranes based on their molecular weight and / or polarity.
3
Revised 8/10/2016
I-C-I
I-C I
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In this experiment you will: 1) Perform an experiment to determine the effect that various alcohols have on the integrity of beetroot membranes 2) Observe the structure of intact beetroot cells by preparing a slide of these cells and observing them microscopically Part 1: The Effect of Different Alcohols on Membrane Permeability Alcohol is an organic solvent that, as you observed, disrupts biological membranes. The question you are exploring in this lab is whether there is a difference in the effect on membranes of different kinds of aiconol. You will be testing the effect of 3 different alcohols (methanol, ethanol and n-Propanol) on the permeability of biological membranes. The structural formulas of these three alcohols are shown below: H H. HH T H C C C-OH H C C-OH H C-OH H H H r-Propanol Ethanol Methanol M.W. o .09502 46.06844 M.W. L M.W. 32.0186 Prior to performing the experiment, identify some of the characteristics that determine permeability: What is the molecular weight (M.W.) of each of these alcohols? Fill in above. Rank these three alcohols from most polar to least polar. What criteria did you use to determine polarity? Methanol- MOst Polar,Prapand because it has 3-H 9raups and these are hddrooholoic. The longerth-e alkul Chain beomes the lesspolar Ethanol propanol teast poar Develop a testable hypothesis about the effect of different alcohols on the permeability of biological membranes based on their molecular weight and / or polarity. 3 Revised 8/10/2016 I-C-I I-C I

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