In this question, you need to discuss the results you obtained from the BSA standards to generate your standard curve.  Are you confident in the absorbance values you measured from the BSA standards? Was there variability between your duplicates? Comment on the shape of your standard curve and note the highest absorbance you would consider for interpolating the concentration of an unknown sample.

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 8.5P
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In this question, you need to discuss the results you obtained from the BSA standards to generate your standard curve. 

Are you confident in the absorbance values you measured from the BSA standards? Was there variability between your duplicates? Comment on the shape of your standard curve and note the highest absorbance you would consider for interpolating the concentration of an unknown sample. 

Absorbance (A540)
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0
5
Absorbance (A540) vs Concentration of protein (mg/ml)
10
15
Concentration of protein (mg/ml)
20
25
-Replicate 1
-Replicate 2
Average between replicate 1/2
Transcribed Image Text:Absorbance (A540) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 0 5 Absorbance (A540) vs Concentration of protein (mg/ml) 10 15 Concentration of protein (mg/ml) 20 25 -Replicate 1 -Replicate 2 Average between replicate 1/2
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