In using an ultracentrifuge, arrange the following steps in correct order A. Turn the knob to the desired length of time to start centrifugation. B. Once the rotor has completely stopped, open the lid and remove the centrifuge tubes. C. Set the desired speed. D. Turn on the centrifuge and let it reach the desired temperature E. Open the lid and place centrifuge tubes of equal weights to opposite sides of the rotor.

In using an ultracentrifuge, arrange the following steps in correct order A. Turn the knob to the desired length of time to start centrifugation. B. Once the rotor has completely stopped, open the lid and remove the centrifuge tubes. C. Set the desired speed. D. Turn on the centrifuge and let it reach the desired temperature E. Open the lid and place centrifuge tubes of equal weights to opposite sides of the rotor.

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter30: Assisting With Minor Surgery

Section: Chapter Questions

Problem 2CR

See similar textbooks

Similar questions

Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.
There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectively
An inoculating loop must be flamed to uniform orange-hot from base to tip A. after culture is transferred to the wire loop but before transfer to new media B. before culture is transferred to the wire loop and after culture is transferred from the wire loop to new media C. after culture is transferred to the wire loop and before culture is transferred from the wire loop to new media D. before culture is transferred to the wire loop
In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL
In this step, wash the cells with ice-cold PBS. Drain the PBS, then add ice-cold lysis buffer. Scrape adherent cells off the dish using a cold plastic cell scraper, then gently transfer the cell suspension into a pre-cooled microfuge tube. Maintain constant agitation for 30 minutes at 4°C. Centrifuge in a microcentrifuge at 4°C. You may have to vary the centrifugation force and time depending on the cell type. Gently remove the tubes from the centrifuge and place on ice, aspirate the supernatant and place in a fresh tube kept on ice, and discard the pellet. Q3: To make sure that you used a similar amount of samples, what important step should be done before proceeding the electrophoresis stage? Once you have determined the concentration of each sample, you can freeze them at -20°C or -80°C for later use or prepare for immunoprecipitation or for loading onto a gel. Q4. Why is it necessary to store the prepared lysates in a very low temperature? PLEASE ANSWER Q3 AND Q4
In the streak plate method, what is the best way to ensure that the loop is cool, after it has been flamed a. no need to cool b. Touch the loop to an uninoculated area of the plate c. Touch the loop to your finger to determine the temperature d. Touch the loop to an inoculated area of the plate
Which of the following is similar to a block of highly purified “jello-like” substance that is not completely solid and has numerous pores throughout its structure? A. Micropipette b. Restriction enzymes c. Agarose d. Negative electrode please give correct answer
1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…
Transfer of Culture from Agar Plate to Agar Slant ____ a.) With your free hand, pick up the sterile nutrient agar slant tube. Remove the cap by grasping the cap with the little finger of the hand that is holding the loop. ____ b.) Raise the lid of a petri plate sufficiently to access a colony with your sterile loop. Pick up the organism. ____ c.) Clean your work area with disinfectant. Allow area to dry. ____ d.) Flame an inoculating loop until it is red-hot. Allow the loop to cool. ____ e.) Remove the loop from the tube and flame the mouth of the tube. Replace the cap on the tube. ____ f.) After transfer is done, flame the loop. ____ g.) Incubate the nutrient agar slant at 37°C for 24–48 hours. ____ h.) Flame the mouth of the tube and insert the loop into the tube to inoculate the surface of the slant, using a zigzag motion. Please provide the answer in order
Here is an image of a UV-irradiation (wavelength 254nm) experiment performed on a plate of E. coll after incubation. Which side of the plate do you think has a zero-minute' negative control? A or B or neither? Explain with proper reasoning.
Based on the growth curve, which ONE of the following best describes what would happen if sample of culture in the presence of drug B is inoculated onto drug-free growth medium? Select one: A. Growth present, but cell count increased by at least 99.9% of the original culture B. No growth after 24 hrs C. Growth at 24 hrs D. Not possible to predict because the culture could either be alive or dead E. Growth present, but cell count reduced by at least 99.9% of the original culture
When testing the efficacy of an antibiotic against bacteria on an agar plate, it is important to spread the bacteria evenly across the plate before you add the antibiotic. Why do the bacteria need to be applied evenly? None of the following are true All of the following are true If you add more bacteria in some areas compared to others, it might obscure the effect of the antibiotic If the area right next to the antibiotic initially received relatively few bacterial cells, that might lead you to overestimate the effectiveness of the antibiotic If the area right next to the antibiotic initially received a relatively large number of bacterial cells, that might lead you to underestimate the effectiveness of the antibiotic