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Indicate how the resolving power is determined and how resolution aff ects image visibility.
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- Indicate how the resolving power is determined and howresolution affects image visibility.Differentiate between transmission and scanning electronmicrosopes in image formation and appearance.Suppose you measure the scanning (4x) field of vision diameter with a stage micrometer and it is 2 mm. If high power is 40X, and gives 10X more magnification than scanning power, how big will the diameter of the field of vision be using high power?
- Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %When using the oil immersion lens, what four procedures can be implemented to achieve the maximum resolution?Give the resolution of eyepiece, scanning objective, LPO, HPO and OIO
- Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?Adjusting the objective aperture is an important step to obtain phase contrast in TEM. Compare the differences in objective aperture operation between diffraction contrast and phase contrast.if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?