INDICATE YOUR RESULTS FOR THE FOLLOWING: 1. Thioglycollate medium: For each organism, indicate the location of growth. Indicate for each organism whether it appears to be an obligate aerobe, an obligate anaerobe, a facultative, or a microaerophilic organism. Clostridium sporogenes Pseudomonas aeruginosa Staphylococcus epidermidis Micrococcus luteus
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- 1.Differentiate between a microaerophile and an aerotolerant organism. 2.Why is resazurin a useful media additive for the study of anaerobes? 3.Why is a GasPak anaerobic jar necessary for the culture of anaerobes on plates of Brewer's anaerobic agar but not in tubes of fluid thioglycollate medium (FTM)? 4.Where in the human body would you expect to find an obligate aerobe? Where would you expect to find an obligate anaerobe?Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?Please help me to find positive or negative results for the Oxidase lab test for microbiology, recognize the species and explain the reason behind the result.
- Support your answer using computation-based judgment. Encircle/box your final computed answer in your solutions along with a declaration of TRUE or FALSE for each given situation. UHT-pasteurized milk sample X which was spread-plated in quadruplicates yielded the following bacterial colony counts: 23, 20, 35 and 41 CFU/plate at a dilution factor of 100. Therefore, the milk sample will pass laboratory standards if the quality control laboratory sets the total microbial limit as: “not to exceed more than 30,000 CFU/mL of milk sample”.Why would the following medium not be considereda chemically defined medium: glucose, 5 grams (g);NH4Cl, 1 g; KH2PO4, 1 g; MgSO4, 0.3 g; yeast extract, 5 g;distilled water, 1 liter? What is aseptic technique andwhy is it necessary?Multiple Matching. For each type of medium, select all descriptions that fit. For media that fit more than one description, briefly explain why this is the case. _______mannitol salt agar a. selective medium _______chocolate agar b. differential medium _______MacConkey agar c. chemically defined (synthetic) _______nutrient broth medium _______brain-heart infusion broth d. enriched medium _______Sabouraud’s agar e. general-purpose medium ________triple-sugar iron agar f. complex medium ________SIM medium g. transport medium
- Need help with the following questions for microbiology 1. Which of the following total magnification (on a compound light microscope) must be used to identify bacterial morphology A. 100 x B. 400 x C. 300 x D. 1000 x 2. You learned about the Bio Safety Level (BSL) categories in Microbiology Lab, which of the following BSL category is safe to processed in a lab on open counter top? A. BSL3 B. BSL2 C. BSL1 D. BSL4 E. BSL1 and BSL2 3. Streak Plate method is used for A. all choices are correct B. used to isolate bacteria from a mixed culture/sample C. is used routinely in Microbiological labs D. is essential tool for isolation and identification of any specific bacteria 4. A selective laboratory growth medium for Gram negative bacteria will allow -------. A. only algae to grow B. only viruses to grow C. only G+ to grow D. only G- to grow 5. You learned that microbes are ubiquitous and they are part of normal flora of various body sites, however which body sites is/are supposed to be…a. What oxygen requirements do your think this bacterium will have (obligate aerobe, obligate anaerobe, facultative anaerobe, aerotolerant anaerobe, or microaerophile), and why? b. You run a thioglycollate test of your isolated bacterium. Based on your response to Part A, which thioglycollate tube best represents the result you would expect for your bacterium? Explain your response. If the result you would expect is not represented, explain what the tube should look like. c. What would you expect to be the result of a catalase test of your bacterium? d. What techniques or specialized equipment, if any, would you need to grow cultures of this bacterium?1. Discuss the three (3) possible ways by which microorganisms may utilize inorganic compounds. Answer this comprehensively and discuss your answer thoroughly.
- Hello, I want you pleaseeee to make a dichotomous key based on provided experiments to identify a an unknown bacterium mixture. It can be E. coli, B. Bulgaris, B subtilis, b aerogenes, m leuteus, s. Aureus, and s epidermisis. Make it based on these possible experiments: phenol red broth, mr/vp test, catalase test, oxidase test, nitrate reduction test, citrate utilization test, Malonate utilization test, decarboxylation test, starch hydrolysis test, dna hydrolysis test, lipid hydrolysis test, gelatin hydrolysis test, urea hydrolysis, SIM medium, triple sugar iron agar test, blood agar test. So far we have run a MAC agar and PEAA plate of the bacteriumhttps://courses.lumenlearning.com/microbiology/chapter/controlling-microbial-growth/ Read this Article about Controlling Microbial Growth and answer the following questions: Which is most effective at removing microbes from a product: sanitization, degerming, or sterilization? Explain. What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name at least two factors that can compromise the effectiveness of a disinfecting agent.TSI Test plz answer all questions Why does TSI contain less glucose than sucrose or lactose? Does an acidic slant indicate that the microbe is a coliform? Explain your answer. What is the function of iron in this medium? Production of H2S by bacteria growing anaerobically in the intestines is common. Some H2S is absorbed into the circulation and some is expelled as intestinal gas. Less commonly, in certain individuals, H2S producing bacteria can live in the plaque built up around their teeth. What undesirable effect might that lead to? conclusion