indicators, key used for/ determines reagents, or key positive result ingredients |1. crystal violet Comments/ Medium/test negative result additional info decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result check this for any quadrant streak- isolation of pure culture of bacteria TSA pigmentation of colonies Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of medium/stab not visible 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts SIM 2. Kovac's in one medium) 3. low % agarose motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red 1. fermentation broth test media (list the specific carbs tested) phenylalanine agar urea agar or broth Methyl red test Vogues Proskauer test nitrate broth oxidase test casein agar gelatin deep lysine iron: lysine deaminase lysine decarboxylasse Pseudo. P agar Pseudo. F agar catalase test Mannitol Salt Agar Blood Agar with CAMP reaction Novobiocin sensitivity Bacitracin sensitivity bile esculin coagulase Lancefeild A Immunochromatograp hy test add any other additional tests here
indicators, key used for/ determines reagents, or key positive result ingredients |1. crystal violet Comments/ Medium/test negative result additional info decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result check this for any quadrant streak- isolation of pure culture of bacteria TSA pigmentation of colonies Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of medium/stab not visible 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts SIM 2. Kovac's in one medium) 3. low % agarose motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red 1. fermentation broth test media (list the specific carbs tested) phenylalanine agar urea agar or broth Methyl red test Vogues Proskauer test nitrate broth oxidase test casein agar gelatin deep lysine iron: lysine deaminase lysine decarboxylasse Pseudo. P agar Pseudo. F agar catalase test Mannitol Salt Agar Blood Agar with CAMP reaction Novobiocin sensitivity Bacitracin sensitivity bile esculin coagulase Lancefeild A Immunochromatograp hy test add any other additional tests here
Microbiology for Surgical Technologists (MindTap Course List)
2nd Edition
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Margaret Rodriguez, Paul Price
Chapter3: The Microbiology Laboratory
Section: Chapter Questions
Problem 1TBP
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Transcribed Image Text:indicators, key
used for/ determines reagents, or key positive result
ingredients
1. crystal violet
Comments/
additional info
Medium/test
negative result
decolorization is key. Must
include +/- controls on
gram reaction and size and 2. iodine morfdant
Gram stain
stains purple
stains pink
3. gram's declorizer
4. safranin
shape
every slide to verify result
quadrant streak- isolation
of pure culture of bacteria
check this for any
pigmentation of colonies
TSA
Eosin methylene blue
agar (EMB)
1. sulfur/H2S
2. indole/tryptophasnase
3. motility/flagella
1. blackening of medium
2. red color in added
reagent
3. fuzzy appearance of
1. no blackening of
medium
2.same color of added multi test medium (3 results
reagent (not red)
3. stab is visible an
defined with growth
1. ferrous salts
2. Kovac's
SIM
in one medium)
3. low % agarose
medium/stab not visible
motility agar
Brewer's plate in
Anaerobe jar
Fluid thioglycollate
(FTM)
Citrate slant
phenol red
fermentation broth
test media (list the
specific carbs tested)
phenylalanine agar
urea agar or broth
Methyl red test
Vogues Proskauer test
nitrate broth
oxidase test
casein agar
gelatin deep
lysine iron:
lysine deaminase
lysine decarboxylasse
Pseudo. P agar
Pseudo. F agar
catalase test
Mannitol Salt Agar
Blood Agar with CAMP
reaction
Novobiocin sensitivity
Bacitracin sensitivity
bile esculin
coagulase
Lancefeild A
Immunochromatograp
hy test
add any other
additional tests here
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