Introduction to Experiment 2: Protein Digestion by Pepsin In this experiment, we will look at for evidence of protein digestion using albumin, a protein, and Biuret reagent. Biuret reagent is used to test for the presence of proteins, or more specifically, peptide bonds. When long polypeptide chains are present the solution will turn violet, and when shorter polypeptide chains are present the solution will turn a lighter pink color. What is/are the end product(s) of protein digestion? ____________________________________________________________ What is the purpose of the enzyme pepsin? _______________________________________________________________________ If protein digestion has occurred, will the solution be violet or pink (choose one)? _________________
Introduction to Experiment 2: Protein Digestion by Pepsin
In this experiment, we will look at for evidence of protein digestion using albumin, a protein, and Biuret reagent. Biuret reagent is used to test for the presence of proteins, or more specifically, peptide bonds. When long polypeptide chains are present the solution will turn violet, and when shorter polypeptide chains are present the solution will turn a lighter pink color.
- What is/are the end product(s) of protein digestion? ____________________________________________________________
- What is the purpose of the enzyme pepsin? _______________________________________________________________________
- If protein digestion has occurred, will the solution be violet or pink (choose one)? _________________
Hypotheses/Predictions (See Methods section for details):
- What color do you expect the solution to turn when the P tubes are mixed with Biuret solution and why?
P1 _____________________________________________________________________________________________________________________
P2 _____________________________________________________________________________________________________________________
P3 _____________________________________________________________________________________________________________________
P4 _____________________________________________________________________________________________________________________
- The least protein digestion (or none at all) will take place in tubes (P1, P2, P3, and/or P4)? _________________
Why? __________________________________________________________________________________________________________________
What color will these tubes be after the Biuret test? _____________________________________________________________
- Of the remaining two tubes, tube ________ will have more protein digestion than tube _________.
Why? __________________________________________________________________________________________________________________
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