IRECTION: 1) ILLUSTRATE THE SPECTROPHOTOMETRIC WORKFLOW PROCESS STARTING FROM THE LIGHT SOURCE UP TO THE READ-OUT DEVICE. MAKE SURE TO LABE COMPLETELY.

Q: Define stimulated emission depletion (STED) microscopy technique.

A: The organisms or substances that cannot be observed with unaided eyes are microscopic. The equipment…

Q: Draw and label FIVE (5) main components for UV-Vis spectrophotometry. Briefly explain the function…

A: When Lab scientists have to be measured total protein by UV-spectroscopy some important things…

Q: Indicate how the resolving power is determined and how resolution aff ects image visibility.

A: Resolving power of a microscope Apart from producing a magnified image of an object, the objective…

Q: Describe the process of Scanning Confocal Microscopy?

A: Microscopy allows magnification of structures to visualize them.

Q: Artefacts seen in virtual slides of given data below. Microscopy not radiology.

A: Artifacts are seen in imaging techniques as a result of presence of some compound in the tissue or…

Q: Resolution in [mm] Medical imaging modality 1: X-ray projection radiography |2: X-ray CT 3:…

A: The diagnosis of disease is essential as it help in detection of the disease so that it can be…

Q: Explain differential-interference contrast (DIC) microscopy.

A: Microscopy is a technique through which the microscopic objects which cannot be seen through naked…

Q: Define positron emission tomography (PET)

A: A diagnostic tool is an instrument or a device used to diagnose the health status of a body. This…

Q: Discuss the optics of imaging the source onto the retina.

A: The retina is a thin layer of tissue that surrounds the back of the eye on the interior. It is…

Q: Give an example of a typical imaging process for a particular condition and state what radioisotope…

A: Imaging processes involve the use of radioisotopes and can be used for diagnostic purposes. Some of…

Q: What are the light sources used in UV-vis spectrophotometry

A: Spectrophotometer is an instrument used for measuring the concentration of solutes in solution by…

Q: Explain the basic differences between fresh and fixed preparationsfor microscopy and how they are…

A: In microscopy, the preparation of a specimen involves the mounting of a sample on a suitable glass…

Q: What do you mean by deconvolution microscopy technique?

A: The organisms or substances that cannot be observed with unaided eyes are microscopic. The equipment…

Q: Differentiate between bright-phase and dark-phase microscopy in terms of phase shift.

A: INTRODUTION:- brightfield,darkfield and phase contrast are 3 contrast enhancement techniques in…

Q: Compare AAS, AES and UV-Vis spectrophotometer

A: The most sophisticated spectrophotometers are AAS, AES and UV-vis spectrophotometers. They each have…

Q: Please provide the quality assurance procedure that ensures reliable results in CLINICAL MICROSCOPY?…

A: DEFINITION        The maintenance of a desired level of quality in a service or products, especially…

Q: Explain the usage of photoactivated localization microscopy technique.

A: Photoactivated localization microscopy abbreviated as PALM is a microscopy technique that was…

Q: Indicate how the resolving power is determined and howresolution affects image visibility.

A: Resolving power of a microscope is the reciprocal of the minimum distance at which two different…

Q: What is the purpose of confocal microscopy technique ?

A: The microscope instrument is used to view the objects, which are not visible under the naked eye.…

Q: Write down the procedure for millipore filter technique

A: The Millipore filter technique is a new method for the concentration, removal, and enumeration of…

Q: Differentiate the Bright-field and a Dark-field microscopy.

A: Microscopy -- The word microscopy is derived from the Greek word  micros - small Skopeo - to view…

Q: What are the light sources used in UV-vis spectrophotometry.

A: In  case of visible spectrophotometers, the wavelengths measured are in the visible light range…

Q: Explain the Workings of an oil immersion lens.

A: Oil immersion lens: A type of objective lens that can focus when there is a drop of liquid (such as…

Q: How is fluoroscopy different from radiography?

A: Radiography and fluoroscopy are imaging techniques used in the medical studies. The Radiography or X…

Q: What advantages do confocal and deconvolution microscopy provide in comparison to conventional…

A: Confocal microscopy uses light from laser, elimination of background information away from the focal…

Q: Protocol for Mann's methyl blue eosin stain technique

A: The anterior pituitary gland and viral appearance are stained with a polychromatic solution of…

Q: What are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?

A: Fluorescence microscope is a type of microscope that makes use of phosphorescence and fluorescence…

Q: What are the applications of ?contact lenses

A: There are several reasons behind problems that arise in the vision of people. These problems can be…

Q: Differentiate bright-phase and dark-phase microscopy

A: In the course of history of biology, various new tools and techniques have been developed to explore…

Q: Resolution is ______with a longer wavelength of light. a. improved c. not changed b. worsened d. not…

A: Resolution means minimum distance between two distinct points.  It is an important factor in…

Q: Discuss if visual observations match with spectrophotometric analysis results.

A:

Q: What is the difference between low reolution and high resolution?

A: The capability of a microscope to identify details is referred to in microscopy as resolution. To…

Q: Can someone explain the concept behind a sample that lies in the UV range in a spectrophotometer?…

A: UV spectroscopy or UV–visible spectrophotometry refers to absorption spectroscopy or reflectance…

Q: summarize what Atom Probe Tomography is and how it work

A: Answer

Q: Light microscopy enhances and amplifies the refractive index O True O False

A: Advantages : Light microscopes have high magnification. Electron microscopic lens are useful in…

Q: Explain the differences between Brightfield, Darkfield, and Phase-Contrast Microscopy.

A: * Here are some differences between bright field microscopy darkfield miccroscopy and phase contrast…

Q: Explain in detail simple and differential staining and also improving and adjusting contrast in…

A: Staining is a chemical method which is used to colour the organism or the cell so that it is viewed…

Q: 10X)? If it is seen under the objective lens of a. 5X b. 40X с. 100X

A: Objective lens and eyepiece or ocular lens are the parts of a light microscope. Eyepiece is…

Q: Differentiate between transmission and scanning electronmicrosopes in image formation and…

A: In transmission electron microscopy, primary electron beam transmits (passes) through the specimen.…

Q: Exercise 2B (optional)- Field of View and Estimation of Size Calculate the diameter of the field of…

A: Microscopes are a very important tool in the area of scientific research. They allow acquisition and…

Q: What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical…

A: Microscope is the widely used laboratory instrument in the molecular biology, it is mainly used to…

Q: Activity: Calculate the magnification for the following. Actual Snecimen Size Image Size…

A: Magnification is the ability of an instrument to enlarge microscopic images.

Q: What is the stationary and mobile phase of spectrophotometry?

A: Introduction A biotechnique based on the principle of quantitative measurement of light intensity…

Q: What is the main difference between Digital Mammography and Conventional Mammography?

A: A diagnostic tool is an instrument or a device used to diagnose the health status of a body. This…

Q: Define magnification and resolution

A: In biology, i can define the term magnification and resolution on the basis of microscopy.