Is FRAP useful for studying the movement of peripheral membrane proteins on the membrane? Justify your answer.
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- 1. Working as an engineer in the R&D section of a biotech industry, you are asked to select a membrane to concentrate a solution containing a protein with a molecular weight of 200 kDa; Fortunately, it has three types of membrane in the cellar: one for MF, one for UF and one for RO. Which one would you choose to perform the required service? Could you establish some characteristics of the chosen membrane?2. Unlike soluble, cytosolic proteins, transmembrane proteins are more difficult to purify, Which of the following substances is most used to help purify a transmembrane protein? a. high salt solution b. sucrose c. detergent d. ethanol e. glucose6. A carrot, when dried out, becomes soft. There is a way to make it firm and crisp. Explain how and why, using the following guidelines. a) Draw and label a representative cell of the carrot, in its dried state. Be precise in your drawing. b)Describe the type of extracellular solution that is appropriate to add to make the desired change (name and define what type of solution, the net movements of solvent and solutes, etc.). c)Draw a representative cell of the carrot, in its possible state after the change. Represent the movement of water using an arrow to indicate what has happened.
- 1. In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored? 2. How does SDS-PAGE separate proteins and peptides from each other? Explain. 3. Explain the Donnan Membrane Phenomenon. Why is it important for the homeostasis of the cell?4. To demonstrate the separation between the onion cell wall and plasma membrane, carefully lift the cover slip and add a few grains of salt. Return the cover slip and describe what happens.1. When considering tight junctions, adherens junctions, and desmosomes: a. Which ones do not help in resisting mechanical stress and holding cell shape? Why not? b. Considering how the mechanical junctions are placed in the plasma membrane, why don’t they provide a truly sealed barrier?
- 17. The transition temperature of a membrane bilayer is determined directly or indirectly a. the ability of lipid molecules to be packed together b. whether the fatty acid chains of the lipids are saturated or unsaturated c. the extent to which the fatty acid chains of the lipids contain double bonds d. the length of the fatty acid chains. e. All of the above are correct answers.1. What does it suggest when the cell has a lot of intricate smooth endoplasmic reticulum? 2. How are polymers formed in stepwise manner?11. Why is cell membrane said to be selectively permeable? * A. because of the polar nature of tail B. because of the presence of amino alcohol C. because of the presence of phospholipid bilayer D. because of the presence of hydrophobic head and hydrophilic tail
- 1. Imagine a hypothetical situation in which an animal cell such as a hepatocyte or a macrophage would completely lose the ability to produce membrane proteins. a) What would be the structural and molecular organization of such a hypothetical cell? b) How do you think this situation would affect the function of that cell? Be as thorough as you can.3. The protein integrin binds to actin filaments and, by binding to proteins like fibronectins, connects to collagen. In this manner, integrin ______. A.controls the permeability of the cytoplasmic membrane B. suspends organelles within the cytoplasm C. provides a direct linkage between the cytoskeleton and the extracellular matrix D. helps keep individual cells in place and connects adjacent cells to each other1. In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored? 2. Explain the Donnan Membrane Phenomenon. Why is it important for the homeostasis of the cell?
