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Estimate the initial rate, vi, at each initial substrate concentration assuming that only the data obtained during the first 4 minutes are linear. Estimate the KM and Vmax for the saccharase hydrolysis of sucrose.

Estimate the initial rate, vi, at each initial substrate concentration assuming that only the data obtained during the first 4 minutes are linear. Estimate the KM and Vmax for the saccharase hydrolysis of sucrose.

Biochemistry
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter27: Metabolic Integration And Organ Specialization
Section: Chapter Questions
Problem 10P
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NO EXCEL! Please help me to understand to how calculate this exercise without using excel. I'm not allowed to use computer during exam, and I only have learned to calculate these type of exercises by using excel. 

If you can write as much detail as possible I will be thankful.

Exercise 3: Calculations of Enzyme Kinetics Problem 1 The table below shows average values of glucose product concentrations [P] in mM obtained at various times for a pure saccharase (EC 3.2.1.26) acting on sucrose in reactions run at five different initial substrate concentrations [So] in mM. All other reaction conditions, except for the [So] were similar for the reactions. Time (minutes) 0 1 2 4 [So] 10 mM [So] = 15 mM [S] = 25 mM = 0 1.03 2.10 4.04 6.06 0 1.08 2.15 4.22 6.25 0 1.12 2.19 4.30 6.44 [So] = 40 mM 0 1.15 2.23 4.36 6.55 a. Estimate the initial rate, vi, at each initial substrate concentration assuming that only the data obtained during the first 4 minutes are linear. b. Estimate the KM and Vmax for the saccharase hydrolysis of sucrose. c. Comment on the assay design with respect to the [So] and the extents of reaction obtained in the different reactions in relation to the results obtained.
Transcribed Image Text:Exercise 3: Calculations of Enzyme Kinetics Problem 1 The table below shows average values of glucose product concentrations [P] in mM obtained at various times for a pure saccharase (EC 3.2.1.26) acting on sucrose in reactions run at five different initial substrate concentrations [So] in mM. All other reaction conditions, except for the [So] were similar for the reactions. Time (minutes) 0 1 2 4 [So] 10 mM [So] = 15 mM [S] = 25 mM = 0 1.03 2.10 4.04 6.06 0 1.08 2.15 4.22 6.25 0 1.12 2.19 4.30 6.44 [So] = 40 mM 0 1.15 2.23 4.36 6.55 a. Estimate the initial rate, vi, at each initial substrate concentration assuming that only the data obtained during the first 4 minutes are linear. b. Estimate the KM and Vmax for the saccharase hydrolysis of sucrose. c. Comment on the assay design with respect to the [So] and the extents of reaction obtained in the different reactions in relation to the results obtained.
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Is it possible to determine Km and Vmax without using excel? Because we are not allowed to use computer during exam, so I dont understand how its done without using excel?

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Ok, thanks for explaining in details. But I dont understand where you get the values for trend line equation? 0.93 and 0.8936?

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