Q: Why is Sanger sequencing sometimes referred to as "dye-terminator" sequencing?
A: Frederick Sanger and his colleagues created Sanger Sequencing, often known as 'chain termination…
Q: Is the DNA isolated from Cheek Cells using Saline solution, Detergent, and Ice-cold alcohol pure or…
A: DNA is a genetic material present in every cell.
Q: What is the procedure to extract DNA through banana?
A: DNA (deoxyribonucleic acid) is the genetic material of the cell. It is double stranded and is made…
Q: Determine the chemical reagents utilized in banana DNA extraction and their roles. What role does…
A: 3. Liquid dishwashing soap This helps split apart the cell membrane made of lipids and the cell…
Q: what is the difference between DNA microarray and Fluorescence in situ technique? or is the…
A: Difference between FISH and Microarray Technique Fluorescence In Situ Hybridization It is possible…
Q: What does the alcohol do in this experiment? Why does the DNA rise to the top after adding alcohol?…
A: Extraction of DNA from fruits.
Q: What are the benefits of using a mixture classification scheme as outlined in DNA Box 14.1? What…
A: INTRODUCTION German stain commission This commission includes several forensic science and legal…
Q: What is the function of the homogenizing solution in DNA isolation? What are its components?
A: In the process of DNA isolation it is very important to breakdown or lyse the cell this allow the…
Q: Why is the annealing temperature different between samples during DNA extraction & marker gene…
A: Introduction Polymerase chain reaction or PCR laboratort technique by which millions to billions of…
Q: In extracting and Isolating a DNA from yeast and fruit. • What is the role of salt and dishwashing…
A: DNA is the genetic material of most cells and its extraction is necessary for analysis,…
Q: Is the DNA extracted from banana pure? Explain
A: DNA (deoxyribonucleic acid) is the genetic material in all living organisms. DNA exists as a double…
Q: For what purposes is DNA extraction done? (give at least 3 purposes for which you may need to…
A: A technique that is used to isolate DNA from a biological sample is referred to as DNA extraction.…
Q: How many DNA bands would you observe in an ethidium bromide-stained electrophoresis gel if you…
A: In agarose gels and various cesium chloride gra- dient techniques, ethidium bromide is employed to…
Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?
A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…
Q: Using the table below, what volume from the master mix will be dispensed to each sample tube if the…
A: Answer: As we know, C1V1= C2V2, where C1 and C2 = initial and final concentrations V1 and V2 =…
Q: Why are the proteins cleaved into small fragments for protein sequencing?
A: Proteins are the end product of the molecular translation that codes the information present in the…
Q: What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis…
A: DNA is a hereditary molecule found in cells that transmits genetic information from one generation…
Q: How does gel electrophoresis separate DNA fragments of your unknown sample and How will you be able…
A:
Q: For PCR, what property of DNA does temperature influence? Why do the “denaturation” and “annealing”…
A: PCR stands for polymerase chain reaction. It is an in-vitro laboratory technique used for generating…
Q: Why were both “+ DNA” and “- DNA” mixtures prepared and plated?
A: Both “+DNA” and “-DNA” mixtures prepared and plated because in the transformation protocol “+DNA”…
Q: Explain why phenol:chloroform is used in the isolation of genomic DNA (What is the end result and…
A: Ans- When DNA-containing samples are extracted with acidic phenol, the DNA is denaturized, and…
Q: Can DNA be isolated from beef? Discuss the process briefly
A: The purification of DNA from the collected sample of DNA with the help of the various methods and…
Q: What is the function of the chaotropic salt in DNA isolation?
A: These salts are strong protein denaturing agents.
Q: What is the purpose of adding the dishwashing liquid to the DNA extraction?
A: DNA extraction is a method of separating DNA from cell membranes, proteins, and other cellular…
Q: What is the cause of an extra band in a DNA ladder in agarose gel electrophoresis?
A: The following can lead to the formation of an extra band in the DNA ladder lane - 1. Contamination -…
Q: What are the consequences for a DNA sequencing reaction if the ratio of dideoxyribonucleoside…
A: Introduction DNA is composed of nucleotides arranged in a specific sequence. Prior knowledge of DNA…
Q: How do the major and minor grooves in B-DNA compare to those in A-DNA?
A: The Watson-Crick double helix model describes the features of the B form of DNA. However, there are…
Q: What is the purpose of the dideoxynucleotide triphosphates in the Sanger sequencing reaction?
A: In 1977, Sanger proposed chain termination method of DNA sequencing. He used ddNTP or…
Q: What is the principle of Sanger sequencing?
A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…
Q: If you were to isolate genomic DNA from two people and perform restriction enzyme digestion followed…
A: Agarose gel electrophoresis is a technique for distinguishing DNA bands based on size. To…
Q: What are some difficulties with low copy number DNA analysis? What kinds of checks and balances can…
A: INTRODUCTION DNA analysis DNA analysis is a method of analyzing the characteristics of DNA. It is…
Q: Is the DNA soluble in the aqueous solution or alcohol?
A: Solubility of the substance depends on molecular polarity. Polar molecules are hydrophilic…
Q: Consider the following wild-type and mutant sequences:Wild-type ....CTTGCAAGCGAATC....Mutant…
A: Introduction A mutation occurs when the sequence of DNA changes. Mutations can occur as a result of…
Q: Is a faded gel matrix in a DNA ladder in agarose gel electrophoresis still useable for analysis or…
A: Agarose gel electrophoresis is a technique to separate different fragments of DNA on the basis of…
Q: During PCR amplification in preparation for DNA sequencing, why were there different colors at the…
A: Polymerase chain reaction is a process by which millions of copies of a specific DNA segment are…
Q: When isolating DNA, why is it helpful to control environmental conditions?
A: The ability to extract DNA is of primary importance to study the genetic causes of disease and for…
Q: Why is there a need for replica plating? How is it performed?
A: Hi! Thanks for your question. But as you have posted multiple questions, I am answering the first…
Q: Some recombinant DNA techniques depend on the specific hybridization (or annealing) between two…
A: The invention of recombinant DNA technology was carried out by Herbert Boyer & Stanley & N.…
Q: What is the purpose of adding table salt (NaCl) to the DNA extraction buffer?
A: The deoxyribonucleic acid (DNA) carries the genetic information and is usually present in the…
Q: What is a Sanger Sequencing ?
A: The term DNA sequencing encompasses biochemical methods for determining the order of the nucleotide…
Q: What is a short tandem repeat (STR) and which method do we use to detect it and which part of the…
A: Short-term repetition of tandem (STRs) is a short-lived sequence of DNA (2–6 bp) that accounts for…
Q: What is the primary disadvantage of Sanger sequencing?
A: Sanger sequencing the target DNA sequence is determined by copying it into the fragments of…
Q: What is the results of extracting the DNA of a strawberry?
A: Ripe strawberries are an excellent source for extracting DNA because they are easy to pulverize and…
Q: What advantages does fluorescent labeling offer over radioactive methods of labeling DNA?
A: Fluorescent labeling and radioactive labeling have found wide applications in DNA sequence analysis…
Q: Among all these primer pairs from Primer-Blast, which is the best to use for a PCR reaction and why?
A: The hereditary currency of an organism is the gene. The gene is present in the DNA of an organism.…
Q: What are sticky ends? What is their importance in recombinant DNA technology?
A: A restriction enzyme cuts only double-helical segments that contain a particular nucleotide sequence…
Q: What is the rationale for the salting out technique for DNA extraction?
A: DNA is the genetic material that stores all the genetic information. Extraction of DNA is applied…
Q: What are the different methods of quantifying DNA? How would you determine if your
A: Quantitation of DNA ,methods used for quantifying DNA ---* Quantifying of DNA -- In various methods…
- Is it possible to obtain DNA in cloverleaf configuration? Explain.
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- What special kind of nucleotide is used in the Sanger sequencing procedure?Is the DNA isolated from Cheek Cells using Saline solution, Detergent, and Ice-cold alcohol pure or not?What is the purpose of precipitation? Why should the ethanol/lysate boundary not be disturbed? What information do learn from the results of the DNA extraction?